狼尾草台畜二號對於巨噬細胞RAW 264.7的免疫調節活性

王怡涵

doi:10.6831/TMU.2015.00072

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狼尾草台畜二號對於巨噬細胞RAW 264.7的免疫調節活性

Immunomodulatory activities of napiergrass Taishigrass No.2 (Pennisetum purpureum S.) in RAW 264.7 macrophages

王怡涵 , Masters　　Advisor：陳玉華　　

繁體中文 DOI： 10.6831/TMU.2015.00072

狼尾草；巨噬細胞； LPS細菌內毒素；免疫調節； napiergrass ； macrophage ； LPS ； immunomodulatory

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Napiergrass Taishigrass No.2 (Pennisetum purpureum S.) is one of livestock forage species in Taiwan. Recently, napiergrass is considered as one of natural organic diet materials and used to consume for health promotion. However, the effects of napiergrass on health promotion has not been investigated. The aim of this study was to explore the effects of napiergrass Taishigrass No.2 on the modulation of immunity in RAW 264.7 macrophages and lipopolysaccharide (LPS)-induced RAW 264.7 macrophages inflammation. Napiergrass Taishigrass No.2 freeze dry powder was bought from Baoyuan company (Taichung, Taiwan). Napiergrass Taishigrass No.2 was extracted by 99.8% ethanol or water in room temperature for 24 h and condensed for cell culture used. Cells were treated with in the presence or absence of LPS (100 ng/ml) and ethanol extracts of napiergrass (0, 0.625, 1.25, 2.5 mg/ml) or water extracts (0, 5, 10 20 mg/ml) for 24 h. Ethanol extracts of napiergrass increased MMP-9 activities and MMP-9 mRNA expression, but had no effect on MMP-2 activities, implying that napiergrass may have immunomodulatiotory effects. Ethanol extracts of napiergrass suppressed nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), inducible nitric oxide synthase (iNOS) protein expression and CD14 mRNA expression in LPS-induced RAW 264.7 macrophages, implying that napiergrass may have anti-inflammation activities. However ethanol extracts of napiergrass couldn’t decrease LPS-induced NF-κB DNA binding activity in RAW 264.7 macrophages. In the presence or absence LPS-induced inflammation condition, water extracts of napiergrass increased NO concentration in a dose dependent manner. Under cell-free condition, water extracts of napiergrass showed similar NO results as cell condition. Water extracts had no effect to iNOS protein expression, MMP-9 activities, MMP-2 activities and inflammatory associated mRNA expression. However water of napiergrass extracts increased IL-6 and IL-6 production in RAW 264.7 macrophages. Overall, the results obtained showed that napiergrass have immunomodulatiotory effects by increasing MMP-9 activities, IL-6 and TNF-α production on RAW 264.7 macrophages.

Reference ( 96 ) 〈TOP〉

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