利用LVSS-ASEI-Sweeping線上濃縮技術分離四氫大麻酚與其主要代謝物

蔡毅軒

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利用LVSS-ASEI-Sweeping線上濃縮技術分離四氫大麻酚與其主要代謝物

Separation of △9-tetrahydrocannabinol and its major metabolites by large-volume sample stacking-anion selective exhaustive injection-sweeping

蔡毅軒 , Masters　　Advisor：林宜慧　　

繁體中文

大麻；四氫大麻酚；毛細管電泳；線上濃縮；大體積樣品進樣；陰離子選擇性電動進樣； capillary electrophoresis ； on-line concentration ； cannabis ； tetrahydrocannabinol ； THC ； THC-OH-THC-COOH ； ASEI ； LVSS ； Sweeping

Abstract │ Reference (45) │ Altmetrics

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Marijuana is made by the leaves of Cannabis Sativa. With its accessible and less addictive, it is the most commonly abused drug in the world. △9-tetrahydrocannabinol (THC) is the major psychoactive component in marijuana. 11-hydroxy-△9-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-△9-tetrahydrocannabinol (THC-COOH) are most abundant metabolites in human after metabolism. Here, we describe a large-volume sample stacking-anion selective exhaustive injection sweeping method for the determination of THC and its metabolites. First, phosphate buffer (30 mM, pH 2.5) containing 40 % methanol and 100 mM SDS are filled into an uncoated fused silica capillary, and then high conductivity phosphate buffer (200 mM, 0.5 psi, 1 min, pH 2.5) was followed. Samples were loaded by pressure injection (0.5 psi, 3 min) before electrokinetic injection (-2.5 kV, 6 min). The stacking step and separation were performed at -15 kV. The detection wavelength was set at 214 nm. During method validation, calibration polts were linear (r≧0.9964) over a range of 40~6000 ng/mL for THC and THC-OH, and 2~300 ng/mL for THC-COOH. The limits of detection (LODs, S/N=3) were 10 ng/mL for THC, 5 ng/mL for THC-OH, and 500 pg/mL for THC-COOH. Comparing with micellar-electrokinetic chromatography, the results indicated that this stacking CE method could increase about 2000-fold sensitivity for the analysis of THC-COOH. After optimization, this method was feasible to detect THC and it’smetabolites in human urine. It can provide advantages such as simplicity, rapidity, and high sensitivity for the analysis of THC.

Reference ( 45 ) 〈TOP〉

2. Robson, P. J., Therapeutic potential of cannabinoid medicines. Drug testing and analysis 2013.
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3. Kogan, N. M.; Mechoulam, R., Cannabinoids in health and disease. Dialogues in clinical neuroscience 2007, 9, 413-30.
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4. Kalant, H., Adverse effects of cannabis on health: an update of the literature since 1996. Progress in neuro-psychopharmacology & biological psychiatry 2004, 28, 849-63.
連結：
6. Huestis, M. A., Pharmacokinetics and metabolism of the plant cannabinoids, delta9-tetrahydrocannabinol, cannabidiol and cannabinol. Handbook of experimental pharmacology 2005, 657-90.
連結：
8. Del Mar Ramirez Fernandez, M.; De Boeck, G.; Wood, M.; Lopez-Rivadulla, M.; Samyn, N., Simultaneous analysis of THC and its metabolites in blood using liquid chromatography-tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2008, 875, 465-70.
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