探討高自組裝性之短胜肽序列對膠原蛋白模擬胜肽摺疊和自組裝性質影響

林駿達

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探討高自組裝性之短胜肽序列對膠原蛋白模擬胜肽摺疊和自組裝性質影響

The Effects of High Self-Assembled Oligopeptides on the Folding and Self-Assembly of Collagen Mimetic Peptides

林駿達 , Masters　　Advisor：洪嘉呈　　

繁體中文

膠原蛋白；細胞毒性實驗；自組裝；三股螺旋； Collagen ； Cytotoxicity experiment ； Self-assemble ； Triple helix

Abstract │ Reference (38) │ Altmetrics

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Collagen, the most abundant protein in mammals, has been widely used in biomedical materials. In order to increase the structural stability of collagen, we designed and synthesized the collagen mimetic peptides (CMPs) in which an oligopeptide with a high self-assembly propensity was attached. By this design, we expected that the oligopeptide could stabilize the collagen triple helix and assist their self-assembly into higher order structures.
Since some of the CMPs containing Ab(16-22) sequence showed the ability to inhibit the aggregation of A protein in our previous studies, in the first part of this study, we further investigated their cytotoxicity by MTT assay. The results indicate that these CMPs are actually toxic to a Neuro cell N2a.
Therefore, in the second part, we chose another oligopeptide CILFWG as an attachment to CMPs. From this design, we synthesized a series of CMPs : CILFWG(POG)7 , (POG)7CILFWG, and OG(POG)4CILFWG. The collagen peptide (POG)7 and CILFWG peptide were also synthesized for comparison. We used UV-VIS spectroscopy, TEM, and CD to characterize these peptides. The results showed that the oligopeptide could stabilize the collagen triple helices and assist their self-assembly into higher order structures. In particular, this oligopeptide has a more pronounced effect on CILFWG(POG)7 than any other peptide studied. Thus, this oligopeptide could be potentially useful in designing stable collagen assemblies and related biomaterials.

Reference ( 38 ) 〈TOP〉

1. 張峻銘.(2014) 膠原蛋白模擬胜肽對貝它類澱粉蛋白Ab(16-22)聚集探討,碩士學位論文,清華大學化學研究所.
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2. Bella, J., Brodsky, B., and Berman, H. M. Hydration structure of a collagen peptide. Structure 1995;3:893-906
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3. Selkoe, D. J. Cell biology of protein misfolding : the example of Alzheimer's and Parkinson's diseases. Nat Cell Bio 2004;6:1054-61
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4. Stefani, M., and Dobson, C. Protein aggregation and aggregate toxicity : new insights into protein folding, misfolding diseases and biological evolution. J Mol Med 2003;81:678-99
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5. Truant, R., Atwal, R. S., Desmond, C., Munsie, L., and Tran, T. Huntington's disease : revisiting the aggregation hypothesis in polyglutamine neurodegenerative diseases. FEBS J 2008;275:4252-62
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