兩性高分子肝素之合成與塗佈於高分子醫材表面特性之研究

Rapid Formation of Biocompatible Heparin-Coated Surface on PVC: Method, Characterization and its Application

李嘉哲(Jia-Jhe Li)

指導教授 ： 林政鞍

中原大學/工學院/生物醫學工程研究所/碩士(2018年)

https://doi.org/10.6840/cycu201800759

摘要

抗凝血塗層於現代科技上佔有非常重要之地位，其原因主要由於醫療行為上之醫療器材與血液中蛋白接觸時因親疏水性差異而引起血栓效應，醫療器材表面引起血栓時容易造成使用效率降低且安全性有疑慮。現代抗凝血塗層中以肝素改質此技術最廣為大眾研究，肝素本身為人體血管內壁糖萼層中的主要醣蛋白結構之一且存在於血管內皮層中作為保護功用，其親水性與生物相容性在材料的選擇上具有非常多的優點。在此研究中我們提出三種兩性肝素高分子之製作，利用電性吸附方式快速合成出兩性高分子且使肝素分子結構上同時含有親水性與疏水性，拉伸塗佈之塗佈方式快速塗佈於高分子表面(例：聚氯乙烯)，且塗佈後表面能改善原高分子表面之疏水性，為了對高分子塗佈後定性與定量高分子於表面，於本實驗中我們利用甲苯胺藍染劑對高分子進行染色，同時利用吸收光譜儀對表面進行定量分析，從中我們發現兩性高分子會因拉伸速度與分子結構不同造成表面高分子塗佈之定量與定性的差異性，而兩性高分子塗佈後表面會受到溫度與鹽類效應的影響，從中我們發現當給予溫度時高分子定量值會提高，表面高分子受到溫度影響使親水端與疏水端於表面翻轉同時使表面親水性提高；鹽類效應加入時因鹽濃度的高低會使高分子表面產生不同的結果，當鹽類濃度1%時能適當使表面高分子部分疏水端脫離使表面更親水；當鹽濃度高至20%會使表面高分子完全被鹽影響而造成脫附且兩性高分子可塗佈於任意表面達到肝素改質之技術應用。

關鍵字

肝素 ； 醣萼 ； 兩性高分子 ； 肝素改質 ； 物化特性

並列摘要

Anticoagulant coating plays an important role in modern technology, this due to induction of the thrombogenicity that caused by different hydrophobicity when medical devices come in contact with blood protein which can impair the performance of implantable medical devices and potentially cause serious adverse clinical events. Therefore biological coating with anticoagulant function have been developed. Heparin coating is widely studied for modern anticoagulant coating. Heparin is one of the major glycoprotein structures in the glycocalyx of inner human blood vessels. It has advantages of hydrophobicity and biocompatibility. Commonly, there are two kinds of heparin modifications: electrical adsorption and covalently bonding. Two types of heparin modification used commercially: the last contact method and the polymer cross-linking method. In this study, we proposed three kinds of amphiphilic heparin modification. That was rapidly synthesized with electric adsorption which the modified heparin molecular structure that has both hydrophilic and hydrophobic ends, then conjugated to polymer surface (such as, PVC) with dip coating method. It improved the hydrophobicity of the polymer surface. In order to characterize and quantify the coated polymer, in this experiment, we dyed the polymer with Toluidine Blue dye and measured using absorption spectrometer. From this we found that the amphiphilic heparin will cause quantitative and qualitative differences in surface polymer due to different stretching speed and molecular structure, also the surface of the amphoteric polymer was affected by temperature and salt , which we found when the temperature is risen, the quantitative value of the polymer will increase, as the hydrophilic and hydrophobic ends were turned over and increased the surface hydrophobicity. Meanwhile, addition of the salt can modify the hydrophobic strength of hydrophobic ends, thus leads to detachment of hydrophobic end of the surface to enhance the surface hydrophobicity, providing biocompatibility for cell attachment and proliferation. The amphiphilic polymer can be applied to any surface for application of heparin modification.

並列關鍵字

Hepari ； Glycocalyx ； Amphiphilic heparin ； Heparin coating ； Physicochemical properties

參考文獻

參考文獻

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