鹼性抗生胜肽Indolicidin及其類似物對於人 類皮膚細胞及人類表皮皮膚癌細胞之影響

Biological Activities of Indolicidin and Its’ Analogues on Fibroblast, Keratinocyte and Human Epidermoid Carcinoma

林昭呈(Zhao-Cheng Ling)

指導教授 ： 謝瑞香

中原大學/工學院/生物醫學工程研究所/碩士(2011年)

https://doi.org/10.6840/cycu201100070

摘要

許多文獻指出，從牛的嗜中性白血球中所分離出來的鹼性抗生胜
肽Indolicidin 具廣效抗菌性，可對抗真菌、細菌或病毒。越來越多研
究發現一些鹼性抗生胜肽其實也存在對癌細胞的抗癌活性，因此本實
驗想探討Indolicidin 及其類似物IL-K7、IL-F89 及IL-K7F89 是否會
對人類表皮膚癌細胞產生抗癌活性或對正常皮膚細胞存在細胞毒
性。
實驗希望找出適合的濃度與胜肽，對皮膚癌細胞具高抗癌活性並
且對正常皮膚細胞擁有低細胞毒性，所以本實驗選用三種濃度：60μM、
30μM 和10μM 來進行實驗，並對利用PI 染色及TUNEL assay 來觀察
細胞受胜肽作用後是經由凋亡或是壞死而導致死亡。
由實驗結果得知10μM 的IL-K7F89 有高抗癌細胞活性能抑制人
類表皮皮膚癌細胞的生長，只對角質細胞及纖維母細胞產生些微的細
胞毒性。由PI 染色和TUNEL assay 結果得知，IL 及其類似物會造成
細胞壞死並非凋亡。之前研究也證實IL-K7F89 具有比IL 高的抗菌活
性也具備比IL 低的溶血活性，結合這些優點或許未來可將其應用在
治療表皮皮膚癌細胞上。

關鍵字

抗癌活性 ； 鹼性抗生胜肽 ； Indolicidin

並列摘要

Many literatures indicated that indolicidin(IL), one of cationic
antimicrobial peptides(CAPs) isolated from the cytoplasmic granules of
bovine neutrophils, has broad-spectrum antimicrobial activity. It can
inhibit the growth of bacteria, fungi and even viruses. A growing number
of studies have shown that some of cationic antimicrobial peptides may
own cytotoxic activity against cancer cells. We, therefore, try to
investigate the cytotoxic activity of IL and its analogues against human
epithelial carcinoma A431and normal human skin cells, HS68 and
CRL2309.
We vary the concentration of IL and its analogues and investigate
the relationship between peptide concentration and cell toxicity. The
suitable concentrations may be found to have high activity against human
epithelial carcinoma but low cytotoxicity toward normal fibroblasts and
keratinocytes. Three concentrations were tested: 60μM, 30μM and 10μM.
The result showed that one of the IL analogue, IL-K7F89, has the highest
anticancer activity to A431 and the lowest cytotoxic activities toward
HS68 and CRL2309 at 10μM. By using PI stain and TUNEL assay, we
also found that indolicidin and its analogues kill cells by direct necrosis
but not inducing apoptosis. In addition, previous study had indicated that
IL-K7F89 has higher antimicrobial but lower hemolytic activity than IL
does. Our study further indicated that the IL analogue, IL-K7F89, will
also be a good candidate for cancer treatment.

並列關鍵字

activity to against ； cationic antimicrobial peptide ； Indolicidin

參考文獻

Journal of Structural Biology, 154(1) (2006) 42-58.

[8] Yoon, W. H., Park, H. D., Lim, K., Hwang, B.D., Effect of

[26] Yang Sung-Tae, Shin Song Yub, HahmK Kyung-Soo, Design of

[32] Chilukuri Subbalakshmi, Narasimhaiah Sitaram, Mechanism of

Cell Interaction and Recognition: Therapeutic Aspects, Harwood