合成 Fe3O4，Fe3O4/SiO2，Fe3O4/TiO2 奈米粒子及其應用研究

Synthesis of Fe3O4，Fe3O4/SiO2，Fe3O4/TiO2 nano-particles and Its Applications

沈明宗(Ming-Zong Shen)

指導教授 ： 王宏文

中原大學/理學院/化學研究所/碩士(2007年)

https://doi.org/10.6840/cycu200700489

摘要

本論文提出以最簡單、快速的化學反應，合成Fe3O4奈米磁性粒子之研究。本實驗利用微波水熱法合成 Fe3O4 奈米磁性粒子，和藉由改變濃度來控制粒子大小，再利用Stober process成功製備 Fe3O4/SiO2 的奈米核/殼粒子。
以微波水熱法合成 Fe3O4 磁性奈米微粒，並利用磁性奈米微粒優異的生物之相容性、超順磁特性、低毒性與生物催化特性等優點，將其應用於拋棄式葡萄糖生物感測器，以期提高酵素電極之活性面積或促進電子傳遞之能力，進而提升感測器之靈敏度。拋棄式酵素電極利用 Fe3O4 奈米微粒以及葡萄糖氧化酵素滴覆於金平板電極上製備而得。
本研究利用循環伏安法，探討酵素電極於修飾過程中各修飾物質於感測系統所扮演之角色。
本研究利用二氧化矽或二氧化鈦修飾 Fe3O4 奈米粒子，來將DNA純化分離，再藉由洋菜膠體電泳分析。

關鍵字

Fe3O4 ； 生化感測器 ； 生化分離

並列摘要

Synthesis of Fe3O4 magnetic nanoparticles is carried out by microwave hydrothermal method. The size of Fe3O4 magnetic nanoparticles is controlled by changing solution’s concentration. We have prepared the Fe3O4/SiO2 Core/shell nanoparticles by using Stober process.

In this study, Fe3O4 magnetic nanoparticles prepared by microwave hydrothermal method and is used for disposable glucose biosensor. The advantages such as excellent biocompatibility, low toxicity, superparamagnetic and biocatalytic properties of Fe3O4 nanoparticles was expected to increase electrode’s active area or promote electrons transfer, and hence improve the sensitivity of the biosensor. The disposable glucose electrodes were constructed by simple surface coatings of GOD and mixture of Fe3O4 nanoparticles onto gold electrodes.

In this study, the modified processes of the gold electrodes and the related electrochemical characteristics were analyzed by cyclic voltammetry (CV).Purification and separation the DNA by the Fe3O4 nanoparticles modified with SiO2 or TiO2 was characterized by agarose gel electrophoresis.

並列關鍵字

biosensor

參考文獻

20.B. Fang, G. Wang, W. Zhang, M. Li and X. Kan. ,”Fabrication of

55.Nelcy D. S. Mohallem, Luciana M. Seara, Miguel A. Novak, and Elis H. C. P. Sinnecker, “Magnetic Nanocomposite Thin Films Prepared by Sol-Gel Process”, Brazilian Journal of Physics, 36 [3B] 2006

2.Frank Caruso, “Nanoengineering of Particle Surfaces”, Adv. Mater. 2001,13,NO.1 p.11-21

4.S.M. Marinakos, et.al., and D.L. Feldheim, “Template Synthesis of One-Dimensional Au, Au-Poly(pyrrole), and Poly(pyrrole) Nanoparticle Arrays” ,Chem. Mater. 10 (1998) 1214-1219

5.S.M. Marinakos, et.al., and D.L. Feldheim,”Gold particles as templates for the synthesis of hollow polymer capules.Control of capsule dimensions and guest encapsulation”, J. Am. Chem. Soc, 121 (1999) 8518-8522