開發新型抗菌塗層於骨科移植材料上之應用

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doi:10.6840/cycu202100408

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開發新型抗菌塗層於骨科移植材料上之應用

Development of new antibacterial coating for orthopedic transplantation materials

詹皓群 , Masters　　Advisor：吳瑞璋　　Co-advisor ：黃郁慈

繁體中文 DOI： 10.6840/cycu202100408

抗菌胜肽；大腸桿菌；金黃色葡萄球菌；膜破壞； PEEK改質； AMPs ； E. coli ； S. aureus ； Membrane destruction ； Modified PEEK

Abstract │ Reference (128)

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

This study reports the antibacterial capability of several types of Antimicrobial peptides (AMPs) against Staphylococcus aureus (S. aureus, a gram positive bacterium) and Escherichia coli (E. coli, a gram negative bacterium) and also investigates if AMPs-coating films are applicable on the materials for transplantation.
The IC50 (half maximum inhibitory concentration) and MIC (minimum inhibitory concentration) of all AMPs candidates were calculated using the results of their visible light OD values and AlamarBlue fluorescence readings. Two AMPs candidates, D-GL13K and MSI-594, were concluded effective in inhibiting both gram positive and negative bacteria. The data obtained for D-GL13K against E. coli were IC50 3 μg/ml and MIC 3.5 μg/ml and against S. aureus IC50 1.7 μg/ml and MIC 2.5 μg/ml. The data obtained for MSI-594 against E. coli were IC50 1 μg/ml and MIC 4 μg/ml and against S. aureus IC50 6 μg/ml and MIC 7 μg/ml.The damage mechanism of bacteria surface was observed by FE-SEM. It was concluded that bacteria in tests were inhibited by membrane destruction. The surface modification of PEEK（Polyetheretherketone）by EDC (1-ethyl-(3-dimethylaminopropyl) carbondimimide) was also investigated. Its surface became rough by FE-SEM observation. The antibacterial activity of modified PEEK was tested. The results showed that the mixed coating of MSI-594 and EDC still maintained the antibacterial activity against E. coli.
This study concludes the inhibiting effectiveness of D-GL13K and MSI-594 against test antibacterial and also the potential feasibility of surface modification of PEEK for transplantation. This study could be extended to the surface modification of PEEK using an AMPs coating enhanced its stability by EDC. That modification could improve the antibacterial capabilities of materials for implantation from the risks of infection.

Reference ( 128 ) 〈TOP〉

[1] L. J. Zhang and R. L. Gallo, "Antimicrobial peptides," Curr Biol, vol. 26, no. 1, pp. R14-9, Jan 11 2016, doi: 10.1016/j.cub.2015.11.017.
[2] R. J. Dubos, "Studies on a bactericidal agent extracted from a soil bacillus: I. Preparation of the agent. Its activity in vitro," The Journal of experimental medicine, vol. 70, no. 1, p. 1, 1939.
[3] J. G. Hirsch, "Phagocytin: a bactericidal substance from polymorphonuclear leucocytes," The Journal of experimental medicine, vol. 103, no. 5, p. 589, 1956.
[4] P. Bulet, R. Stöcklin, and L. Menin, "Anti‐microbial peptides: from invertebrates to vertebrates," Immunological reviews, vol. 198, no. 1, pp. 169-184, 2004.
[5] R. M. Epand and H. J. Vogel, "Diversity of antimicrobial peptides and their mechanisms of action," Biochimica et Biophysica Acta (BBA)-Biomembranes, vol. 1462, no. 1-2, pp. 11-28, 1999.

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