氧化鋅奈米線指叉電極晶片進行血球阻抗量測之研究

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氧化鋅奈米線指叉電極晶片進行血球阻抗量測之研究

Study on Impedimetric Measurement of Blood Cells Using ZnO-Nanowire Interdigitated Electrodes

黃義中 , Masters　　Advisor：張耀仁　　

繁體中文

氧化鋅奈米線；指叉電極； ZnO nanowires ； IDE

Abstract │ Reference (25) │ Altmetrics

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Abstract
In this thesis, a printed circuit boards (PCB) was treated as the chip substrate to allow FR4 copper foil to be patterned into copper interdigitated electrodes by photolithography process. Different types of photoresists were utilized to define the location of Zinc oxide (ZnO) nanowire selectively into the gap of copper interdigitated electrodes. Electrochemical impedance of erythrocytes was detected and analyzed via impedance meter and the recorded Nyquist plot. The results show that the electrochemical impedance spectra are distinguishable and more sensitive in comparison with those of interdigitated electrodes without ZnO nanowire. ZnO nanowire interdigitated electrodes can be exercised to detect the impedance of 3% red blood solution with a trace volume of 0.2ul. In different volume levels, the value can still be detected. The increase of probe volume provides larger impedance value which indicates the blood cells have larger impedance than the solvent.
The impedance value of blood cell can be obtained from measuring with the nanowire interdigitated electrodes system. A simulated equivalent circuit of resistor(s) and capacitor(s) can be derived from the recorded Nyquist plot.  Following with the measured value of solution resistance and the Electrical Double-Layer capacitance, the impedance of red blood cells can be calculated by deduction.

Reference ( 25 ) 〈TOP〉

[1] Y. Zhao, P. Deng, Y. Nie, P. Wang a, Y. Zhang, L. Xing and X. Xue,“Biomolecule-adsorption-dependent piezoelectric output of ZnO nanowire nanogenerator and its application as self-powered active biosensor,” Biosensors and Bioelectronics, Vol .57,pp. 269–275, 2014.
連結：
[2] J.Y. Lee, C.Y.Wang, C.F. Huang and A.T. Cheng, “Interdigitated Electrodes Based on Impedance Biosensor for Sensing Peptide LL-37,” IEEE EMBS,pp. 71-74, 2011.
連結：
[3] S.N.S. A. Ayob and U. Hashim, “The synthesis and fabrication of Titanium dioxide nanowires-based biosensor,” IEEE-ICSE,pp.145-148, 2012.
連結：
[4] Y. Hea, W. Zhangb, S. Zhanga, X. Kanga, W. Penga and Y. Xua, “Study of the photoconductive ZnO UV detector based on the electrically floated nanowire array,” Sensors and Actuators A, Vol.181, pp.6-2,2012.
連結：
[5] D. Meng, N.M. Shaalan, T. Yamazaki and T. Kikuta, “Preparation of tungsten oxide nanowires and their application to NO2 sensing,” Sensors and Actuators B, Vol.169, pp.113– 120, 2012.
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