青鱂魚仔魚體表離子細胞的排氨參與鈉離子吸收機制

吳淑貞

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青鱂魚仔魚體表離子細胞的排氨參與鈉離子吸收機制

Ammonia-dependent Na+ uptake in mitochondria-rich cells of medaka (Oryzias latipes) larvae

吳淑貞 , Masters　　Advisor：林豊益　　

英文

離子細胞；富含粒線體細胞；鈉氫交換蛋白；鈉離子吸收；氨；青將魚；掃瞄式離子選擇電極技術； ionocyte ； mitochondria-rich cell ； Na+/H+ exchanger ； Na+ uptake ； Rhesus glycoprotein ； ammonia ； medaka ； scanning ion-selective electrode technique

Abstract │ Reference (53) │ Altmetrics

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The mechanisms of Na+ uptake and NH4+ excretion at gills of freshwater fish have been studied for decades but the detail remains unclear. To investigate the mechanisms, a scanning ion-selective electrode technique (SIET) was applied to detect the H+, Na+, and NH4+ activities and fluxes at the skin surface of newly-hatched medaka larvae. By probing the ionic fluxes at specific cells in the skin, MRCs were found to be the major sites for Na+ uptake and NH4+ excretion. However, H+ probing at MRCs revealed two groups of MRCs: acid-secreting MRCs (MRC+) and base-secreting (probably NH3) MRCs (MRC-). Treatment with EIPA (100 μM) respectively blocked H+ excretion, NH4+ excretion, and Na+ uptake by 22%, 35%, and 54 %, suggesting that the Na+/H+ exchanger (NHE) is involved in H+, Na+, and NH4+ transport. Low-Na+ water (< 0.001 mM) or high-NH4+ water (5 mM) acclimation caused more MRC- appearing in skin surface, and simultaneously increased Na+ uptake and NH4+ excretion but decreased or even reversed the H+ gradient at the skin and the H+ flux at MRCs. Raising the external NH4+ significantly blocked NH4+ excretion and Na+ uptake, but increased the H+ gradient at the skin. In contrast, raising the acidity of the water (pH 7 to pH 6) enhanced NH4+ excretion and Na+ uptake by MRCs while the H+ activity at the apical surface of MRCs was reduced. The correlation between NH4+ production and H+ consumption suggests that MRCs excrete non-ionic NH3 (base) by an acid-trapping mechanism. The present study suggests a Na+/NH4+ exchange pathway in apical membranes of MRCs, in which a coupled NHE and Rhesus (Rh) glycoprotein is involved, and the Rh glycoprotein may drive the NHE by generating H+ gradients across apical membranes of MRCs.

Reference ( 53 ) 〈TOP〉

Biver, S., Belge, H., Bourgeois, S., Van Vooren, P., Nowik, M., Scohy, S., Houillier, P., Szpirer, J., Szpirer, C., Wagner, C. A. et al. (2008). A role for Rhesus factor Rhcg in renal ammonium excretion and male fertility. Nature 456, 339-43.
連結：
Boisen, A. M., Amstrup, J., Novak, I. and Grosell, M. (2003). Sodium and chloride transport in soft water and hard water acclimated zebrafish (Danio rerio). Biochim Biophys Acta 1618, 207-18.
連結：
Braun, M. H., Steele, S. L., Ekker, M. and Perry, S. F. (2009). Nitrogen excretion in developing zebrafish (Danio rerio): a role for Rh proteins and urea transporters. Am J Physiol Renal Physiol 296, F994-F1005.
連結：
Bury, N. R. and Wood, C. M. (1999). Mechanism of branchial apical silver uptake by rainbow trout is via the proton-coupled Na+ channel. Am J Physiol 277, R1385-91.
連結：
Choe, K. P., Kato, A., Hirose, S., Plata, C., Sindic, A., Romero, M. F., Claiborne, J. B. and Evans, D. H. (2005). NHE3 in an ancestral vertebrate: primary sequence, distribution, localization, and function in gills. Am J Physiol Regul Integr Comp Physiol 289, R1520-34.
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