半導體量子點於癌細胞螢光標定之研究

Fluorescent Labeling of Cancer Cells with Semiconductor Quantum Dots

陳正龍(Chan-Long Chen)

指導教授 ： 陳家俊

國立臺灣師範大學/理學院/化學系/碩士(2002年)

摘要

無資料

關鍵字

量子點 ； 螢光標定 ； 奈米晶體 ； 半導體

並列摘要

Abstract
Inorganic nanomaterials that interface with biological systems have
recently attracted widespread interests in cellular and biomedical applications.
Semiconductor quantum dots (QDs) are among the most promising emerging
fluorescent labels for cellular imaging. In this thesis, to study the way into
cell、biocompatibility and photostability of QDs, we fluorescently labeled
breast cancer cells ( MCF 7 ) using water-soluble CdSe/ZnS core-shell
nanocrystals. Confocal microscopy images indicated that the cells remained
stably labeled for several days and continued cell division. However, to establish
whether QDs can specifically label organelles at a subcellular level, we used
QDs modified with the nucleus localization signal ( NLS ) peptides to label the
nuclei of the cells. The results showed that much labeling signals are specific for
the nuclei and are brighter and considerably more photostable than comparable
organic dyes. These results encourage the construction of more complex
functional QDs for protein tracking in cell.

並列關鍵字

Quantum dot ； fluorescent label ； nanocrystal ； semiconductor

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