微粒型甲烷單加氧酶研究
－單元B之水層暴露蛋白質與銅離子作用性質

紀丞哲

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微粒型甲烷單加氧酶研究－單元B之水層暴露蛋白質與銅離子作用性質

A Study of the Reconstitution of Copper ions with the Partially Expressed Aqueous Exposed Domain of the Particulate Methane Monooxygenase Subunit B Protein

紀丞哲 , Masters　　Advisor：俞聖法;黃得時　　

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微粒型甲烷單加氧酶；嗜甲烷菌；銅離子的鍵結常數； Methylococcus capsulatus (Bath) ； PmoB ； Copper binding constant ； Aqueous ； Particulate Methane Monooxygenase

Abstract │ Reference (148) │ Altmetrics

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Methylococcus capsulatus (Bath) is one of methanotrophic bacteria.Differential expression of either sMMO or pMMO is regulated by the concentration of copper ions available to the cells. During the past decade, the experiments from either electron paramagnetic resonance (EPR) or X-ray absorption spectroscopy conclude that pMMO is a multicopper protein. The copper ions in the pMMO were classified into C-clusters and E-clusters. The E-clusters found to be associated with water-exposed domains of the 45 kDa subunit which were responsible for channeling electrons to the C-cluster. It would be crucial to shed light on the copper binding affinity on even coordination feature in this
aqueous domain of PmoB subunit to show how the electrons transfered from E-Cluster to C-cluster where the methane oxidation take place. Therefore, over-expressed PmoB subunit, a water-soluble domain of of pMMO in E.coli. system was conducted by using gene engineering technique. Thus, whether partially expressed proteins can be folded properly or the copper ions exhibt capability to sequester can be possilbly deduced. Furthermore, the copper concentration was quantitated with atomic absorption spectroscopy, and the copper binding constant was examessed by Scatchard plot. The results indicated that the copper ion played at least two important roles in both coordinating with protein and stablized the protein folding structure. The maximum number of protein binding copper is about 16.70. In the future, we will express other domains of water-exposed protein to study which sequence has stronger copper binding effect, and resolve their structures and copper coordinations via X-ray crystallography.

Reference ( 148 ) 〈TOP〉

Denitrification associated with Groups I and II methanotrophs in a gradient
連結：
enrichment system. FEMS microbiol. ecol. 1995, 18, 289-298.
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Biology at the University of Bergen (UiB) 2002,
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Diversity of Alkane Hydroxylase Systems in the Environment. Oil & Gas
連結：
4. Chan, S. I., Chen, K. H. C., Yu, S. S. F., Chen, C. L. and Kuo, S. S.
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