脂肪分解酵素Pseudomonas cepacia固定於奈米纖維膜並應用在生質柴油製程之研究

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脂肪分解酵素Pseudomonas cepacia固定於奈米纖維膜並應用在生質柴油製程之研究

Immobilization of Pseudomonas cepacia Lipase onto Nanofibrous Membrane for Biodiesel Production

范逸軒 , Masters　　Advisor：吳文騰　　

繁體中文

生質柴油；固定化技術；脂肪分解酵素；電紡織技術； biodiesel ； immobilization ； lipase ； electrospinning

Abstract │ Reference (48) │ Times Cited (2) │ Altmetrics

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In the study, electrospun polyacrylonitrile (PAN)nanofibrous membrane was activated by amidination reaction for immobilized Pseudomonas cepacia lipase with covalent binding. 
For optimized immobilization, different levels of temperature, pH value, immobilization time and enzyme concentration were investigated to determine the optimal specific activity of enzyme. The reaction conditions were obtained as the temperature at 50°C, pH value at 6, immobilization time of 30 min, and enzyme concentration for immobilized lipase of 0.5 wt%. The biocatalytic reaction between enzymatic active site and substrates can be described by using the Michaelis-Menten equation, so the kinetic parameters of immobilized lipase, Km and Vmax, were determined as 88.42 mM and 18.27 U/mg-protein, respectively. Besides, the thermal and pH stabilities of immobilized lipase were improved and still retained the activity as the original one after 10 times repeated uses.
The effects of methanol amount, water content, temperature and content of fatty acid on the conversion of biodiesel were also illustrated and the optimal operation condition was determined. Under the optimal condition, methanol of 4 g, water content of 3.84 g, temperature at 30°C and 10 g oil (contain 50% fatty acid) for biodiesel production, a conversion of 90% was obtained in 24 hours and can remain the conversion of 81.2% after 10 times repeated uses. This effective immobilized lipase demonstrated the high potential for biodiesel production in industrial applications.

Reference ( 48 ) 〈TOP〉

Anita A, Sastry CA, Hashim MA. Immobilization of urease using Amberlite MB-1. Bioprocess Engineering 17: 355-359, 1997.
連結：
Arroyo M, Sanchez-Montero JM, Sinisterra JV. Thermal stabilization of immobilized lipase B from Candida antarctica on different supports: effect of water activity on enzymatic activity in organic media. Enzyme Microb Technol 24: 3-12, 1999.
連結：
Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72: 248-254, 1976.
連結：
Brzozowski AM, Derewenda U, Dererwenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Blorkling F, Huge-Jensen B, Patkar SA, Thim L. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 351: 491-495, 1991.
連結：
Demirbas A. Importance of biodiesel as transportation fuel. Energy Policy 35: 4661-4670, 2007.
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