以硫醇修飾之金電極及其在尿蛋白生醫感測之應用

曾郁皓

doi:10.6844/NCKU.2012.01317

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以硫醇修飾之金電極及其在尿蛋白生醫感測之應用

Thiol-modified gold electrode and its application on albumin biosensing in urine

曾郁皓 , Masters　　Advisor：楊明長　　

繁體中文 DOI： 10.6844/NCKU.2012.01317

牛血清白蛋白；循環伏安法； 8-巰基辛酸；自組裝單分子層； EDC/NHS ； Bovine Serum Albumin ； Cyclic Voltammetry ； 8-mercaptooctanoic acid ； self-assembly monolayer ； EDC/NHS

Abstract │ Reference (84) │ Altmetrics

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Human urinary albumin played an important role in the earlier stage diagnosis of kidney disease. The self-examination of urine albumin could help patients in determining seriousness of kidney disease. In this study, an electrochemical urinary albumin sensor was prepared. Bovine serum albumin (BSA) was applied as the analytes, and various kinds of thiol were used to form self-assembly monolayers (SAMs) on gold electrode. Cyclic voltammetry (CV) and AC impedance analysis were used to determine peak potential, peak current and impedance in a Fe(CN)63-/Fe(CN)64- system. Fourier Transfer Infrared Spectroscopy (FT-IR) and contact angle goniometer were also used to analyze the bonding between modified electrode and BSA and hydrophobicity of modified electrode.
In preparing sensing electrode, N-(3-Dimethylaminopropyl)-N’- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were modified onto electrode to change the end group of thiols. Peptide form between BSA and thiol during sensing. After cyclic voltammetry, the BSA unbonding to the thiols on the electrode would desorb.
The result showed that redox reactions Fe(CN)63-/Fe(CN)64- on electrodes before immersing in BSA solution were irreversible reactions. After immersed in BSA solution and increasing the BSA concentration, the irreversibility of the redox reaction and the charge transfer resistance increased, and the electrode activity reduced. For 8-mercaptooctanoic acid modified electrode at pH 5.5, the BSA sensitivity was improved from 0.666 ppm-1 (oxidation) and 0.604 ppm-1 (reduction) with bare electrodes to 2.594 ppm-1 (oxidation) and 2.614 ppm-1 (reduction). The detecting upper limit of BSA was 30 ppm. For 12-mrecaptododecanoic acid modified electrode at pH 5.5, although the sensitivity was improved to 13.11 ppm-1 (oxidation) and 11.25 ppm-1 (reduction), the detecting upper limit of BSA decreased to 6 ppm. Since the normal concentration of albumin in urine is 20 ppm, the electrode modified with 8-MOA electrode is proposed the most suitable sensing electrode for the electrochemical urinary albumin sensor.

Reference ( 84 ) 〈TOP〉

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