發展以微液滴系統進行藻類油量檢測之技術

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發展以微液滴系統進行藻類油量檢測之技術

Development of lipid quantification technique for microalgae using microfluidic droplet systems

陳昭名 , Masters　　Advisor：王翔郁　　

繁體中文

微液滴系統； T型微流道；界面活性劑； Nile red ；小球藻； Digital microfluidics ； T-junction ； Surfactant ； Nile red ； Chlorella vulgaris

Abstract │ Reference (43) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Digital microfluidics has been widely applied for biological and chemical research because of its abilities in rapid analysis, high throughput, and independent droplet manipulation. Many parameters affect the formation of droplets such as flow rates, surfactant concentrations, and geometries of microchannels. Manipulating and generating droplets with accuracy are important in obtaining meaningful results. In this study, soybean oil and water (with Tween 20 added in some sets of experiments) were used as continuous and disperse phases, respectively, to create highly monodispersed droplets in a T-junction microchannel. We found that droplets can be generated stably using smooth substrates or adding Tween 20. We have generated monodispersed droplets whose sizes were between 225 (μm) ~107 (μm) when the Capillary number was adjusted from 2.47×10-3 to 4.4×10-1. Adding surfactant decreased the length of droplets under the same flow rates. Furthermore, we obtained a simple relationship between the droplet lengths and the channel width, fluid viscosity and flow rates: L/W_c =1.258φ^0.159 Ca^(-0.081).*
    Following this fundamental research, we used an aquareous solution containing microalgae (Chlorella vulgaris) that have been stained by Nile red as the disperse phase to generate microalgae-containing droplets. We successfully encapsulated various amounts of Nile red labeled microalgae in the droplets. Afterwards, we encapsulated Nile red into droplets to investigate the mixing and extraction of Nile red in a serpentine microchannel. Using this system, future integration of real-time labeling and detection for biological samples can be achieved.
*L: length of droplets; wc: width of channel where the droplet formed; Q= Q_d/Q_c ，Qd: flow rate of disperse phase, Qc: flow rate of continuous phase; Ca= (μ_c v_c)/γ, γ: interfacial tension between continuous and disperse phase, μc: viscosity of continuous phase, vc: velocity of continuous phase.

Reference ( 43 ) 〈TOP〉

1. 謝誌鴻 and 吳文騰, 微藻培養與微藻油脂生產之研究. 國立成功大學化學工程學系博士論文, 2009: p. 1-160.
連結：
2. Chisti, Y., Biodiesel from microalgae. Biotechnology Advances, 2007. 25(3): p. 294-306.
連結：
3. Widjaja, A., C.-C. Chien, and Y.-H. Ju, Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. Journal of the Taiwan Institute of Chemical Engineers, 2009. 40(1): p. 13-20.
連結：
4. 葉俊良 and 吳文騰, 在光生化反應器中以二階段策略培養微藻生產油脂之研究. 國立成功大學化學工程學系碩士論文, 2006: p. 1-91.
連結：
6. Song, H., D.L. Chen, and R.F. Ismagilov, Reactions in Droplets in Microfluidic Channels. Angewandte Chemie International Edition, 2006. 45(44): p. 7336-7356.
連結：

Times Cited (1) 〈TOP〉

陳彥妤(2012)。利用微液滴系統進行螢光偵測改善以及製備微粒子之研究及應用。成功大學化學工程學系學位論文。2012。1-155。

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