亞硼酸─苯基磺醯基化學探針分子之合成及其在醣蛋白微陣列晶片上之應用

劉軒宇

3.232.129.123，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Basic & Applied Sciences > Chemistry
College of Science > Department of Chemistry

Bibliography Management Tool

Related Links

Report an Issue

Search in Library

There is no full-text available at the moment. Airiti would like to request author's authorization. I would like to provide authorization.

亞硼酸─苯基磺醯基化學探針分子之合成及其在醣蛋白微陣列晶片上之應用

Synthesis of Boronic Acid-Tosyl Chemical Probes and It''s Applications in Glycoprotein Microarray

劉軒宇 , Masters　　Advisor：林伯樵　　

繁體中文

亞硼酸；苯基磺醯基；張力促進疊氮-炔環加成反應；微陣列晶片；蛋白質固定化； Boronic acid ； Protein immobilization ； Microarray ； Strain-promoted azide-alkyne cycloaddition ； Tosyl

Abstract │ Reference (67) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Protein microarray is a high-throughput screening platform in the study of protein-protein interaction. However, the orientation and structure integrity of immobilized proteins can considerably affect the corresponding biological activity. To date this end, amino-cyclooctyne and a series of boronic acid (BA)-tosyl chemical probes have been synthesized, and used in the fabrication of glycoprotein microarray. Accordingly, strain-promoted azide-alkyne cycloaddition under homogeneous condition have been shown to improve the efficiency of BA-tosyl chemical probe modification on microarray surface. BA-tosyl functionalized surface can conduct the anchoring of glycoproteins. The boronic acid component recognizes the diols of glycan to form boronate diester ring. The resulting proximity effect can trigger a SN2 reaction by nucleophilic residues of protein to the tosyl group of the chemical probe, thereby immobilizes the protein of interest on microarray surface. In this research, a highly efficient strategy for the immobilization of glycoproteins is proposed and then applied in the study of glycoprotein-protein interactions.

Reference ( 67 ) 〈TOP〉

(1) Zhu, H.; Snyder, M. Curr. Opin. Chem. Biol. 2003, 7, 55-63.
連結：
(4) Griffiths, J. Anal. Chem. 2007, 79, 8833-8837.
連結：
(5) Predki, P. F. Curr. Opin. Chem. Biol. 2004, 8, 8-13.
連結：
(6) Melton, M. Nature 2004, 429, 101-107.
連結：
(7) Hall, D. A.; Ptacek, J.; Snyder, M. Mech. Ageing Dev. 2007, 128, 161-167.
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：