奈米生物感測粒子用於細胞內氧化壓力即時偵測之發展及研究

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奈米生物感測粒子用於細胞內氧化壓力即時偵測之發展及研究

Development of Intracellular Nanobiosensor for Oxidative Stress Detection

杜靜如 , Masters　　Advisor：袁俊傑　　

英文

奈米生物感測粒子；氧化壓力；即時偵測； PEBBLEs ； Sol-gel ； Oxidative stress ； Real-Time

Abstract │ Reference (62) │ Altmetrics

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Balance between oxidants and antioxidants is important to maintain normal cellular functions. Although the most oxidative events are carefully monitored and controlled by the natural defense systems of cells, sustained perturbation of this balance may result in oxidative stress. Oxidative stress-induced damages are usually key factors for aging and many disorders in human cell. In this study, a ratiometric optical PEBBLE (probes encapsulated by biologically localized embedding) nanosensor, which encapsulates catalase and two fluorescent dyes, Ru(II)-tris(4,7-diphenyl-1,10- phenanthroline) chloride ([Ru(dpp)3]2+) and Oregon Green 488-dextran was developed by sol-gel technology for the detection oxidative stress in vivo. The developed ratiometric optical PEBBLE nanosensor is spherical with an average size of 200±50 nm. The catalase encapsulated in the nanosensor is around 8U/mg nanoparticle. Catalase in nanosensor converts H2O2 to oxygen that quenches the fluorescence of [Ru(dpp)3]2+; whereas, the fluorescence of Oregon Green 488-dextran is unaffected, acting as a reference for ratiometric intensity measurement. The developed optical nanosensor exhibits potential for the real-time detection of oxidative stress in living cells.

Reference ( 62 ) 〈TOP〉

1. M. Valko, C.J. Rhodes, Free radicals, metals and antioxidants in oxidative stress-induced cancer Chemico-Biological Interactions 160, 1–40, 2006
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3. J. Chandra, A. Samall, S. Orrenius. Triggering and modulation of apoptosis by oxidative stress. Free Radical Biology & Medicine, 29, 323–333, 2000
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6. M. Valko, M. Izakovic, Role of oxygen radicals in DNA damage and cancer incidence, Molecular and Cellular Biochemistry 266: 37–56, 2004
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7. M. Valko, D. Leibfritz, Free radicals and antioxidants in normal physiological functions and human disease, The International Journal of Biochemistry & Cell Biology, Volume 39: 44-84, 2007
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9. Jose M. Mates, Francisca M. Sanchez-Jimenez, Role of reactive oxygen species in apoptosis: implications for cancer therapy, The International Journal of Biochemistry & Cell Biology, 32: 157-170, 2000
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