近場電紡絲技術應用於排列多種密度、異向性之奈米纖維結構以引導細胞擴散及型態之研究

何哲宇

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近場電紡絲技術應用於排列多種密度、異向性之奈米纖維結構以引導細胞擴散及型態之研究

Guided cell morphology and migration on near-field eletrospun nanofiber-textured substrates with variable local density and anisotropy

何哲宇 , Masters　　Advisor：傅尹坤　　

繁體中文

近場電紡絲技術；選擇性沉積；電壓調節；細胞擴散；褐藻酸鈉； Near-field electrospinning ； Selective deposition ； differential voltage regulation ； cells spreading ； alginate

Abstract │ Reference (34) │ Altmetrics

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This thesis mainly research formation of nanofiber, controlled technology and application in near-field electrospinning. The focus of the study is Direct-write, highly-aligned Alginate-poly(ethylene oxide) nanofiber patterns for cell architecture/ morphology and spreading control. Near-field electrospinning (NFES) has been demonstrated to be able to achieve direct-write and highly-aligned Alginate nanofibers (ANF) with prescribed positioning density. Cells spreading in preferential direction could be observed on parallel aligned nanofibers and the ANF patterns were capable of guiding cell extension, when the distances between them are 10、20、40、80μm, respectively. Alignment of the cells was characterized by elongation and orientation of the cells via the FFT data and. Parallel ANF indicates the alignment values sequentially increased as a function of the positioning density such that incrementally more aligned cells were closely related to the increasing ANF positioning density. And the use of repeated stacking technology to produce three-dimensional nano-fiber structure to observe the fiber for cell reproduction and diffusion These maskless, low-cost and direct-write patterns can be facility fabricated and will be a promising tool to study the cell-based research such as cell adhesion, spreading and tissue architecture.

Reference ( 34 ) 〈TOP〉

[3] Z. M. Huang, Y. Z. Zhang, M. Kotaki, S. Ramakrishna, Composites Science and Technology 63, 2223 (2003).
連結：
[4] D. Sun, C. Chang, S. Li, L. Lin. Nanoletters 6(4), 839 (2006).
連結：
[5] J. D. Schiffman, C. L. Schauer, Biomacromolecules 8, 2665 (2007).
連結：
[8] C. Chang, K. Limkrailassiri, L. Lin, Applied Physics Letters 93, 123111 (2008).
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[14] D. H. Reneker, I. Chun, Nanotechnology 7, 216 (1996).
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