電解水系統於淡水長臂大蝦育苗之應用

謝立帆

doi:10.6346/NPUST.2010.00245

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電解水系統於淡水長臂大蝦育苗之應用

Usage of electrolytic water system in giant freshwater prawn

謝立帆 , Masters　　Advisor：劉俊宏;葉信平　　

繁體中文 DOI： 10.6346/NPUST.2010.00245

水產養殖；電解水；疾病防治；水質改善；育苗系統； aquaculture ； electrolytic water ； disease control ； water quality ； breeding system

Abstract │ Reference (217) │ Times Cited (1) │ Altmetrics

Abstract 〈TOP〉

摘要 學號：M9813012 論文名稱：電解水系統於淡水長臂大蝦育苗之應用   總頁數：88頁 學校名稱：國立屏東科技大學            系(所)別：水產養殖系 畢業時間及摘要別：九十九學年度第二學期碩士學位論文摘要 研究生：謝立帆                        指導教授：劉俊宏 博士 葉信平 博士 論文摘要內容： 本研究之目的係應用電解水系統於淡水長臂大蝦育苗，並分析其作為水產育苗之疾病防治及養殖水處理單元的可行性。本實驗包括分析不同鹽度 15 及 30‰、流速 3 及 6 L/min、功率 1 W 及 5 W 對生成有效氯之影響，結果顯示電解海水有效氯與水之鹽度及電解功率呈正相關，但卻與水流速度呈負相關。電解水對於重要水產病原菌之抑制效果，在 0.5 及 1 mg/L 之有效氯作用 30 及 60 秒後，對六種不同水產病原菌皆具有良好的殺菌效果，包括 Vibrio alginolyticus (宿主:九孔)、V. alginolyticus (宿主:白蝦)、Vibrio vulnificus (宿主:白蝦)、Photobaterium damsela subsp. damselae (宿主:草蝦)、Streptococcus sp. (宿主:龍膽石斑) 及 Vibrio parahemolyticus (宿主:九孔)。另外，電解水去除含氮化合物之效果，在鹽度 15 及 30‰ 時，以電解強度5 W的條件下電解，對於去除養殖水中氨 (NH3-N) 及亞硝酸 (NO2-N) 皆有顯著性的效果，分別在電解 30 及 10 分鐘後，其濃度趨近於 0 mg/L。為瞭解電解水機應用於淡水長臂大蝦育苗之可行性，預先分析其對淡水長臂蝦苗毒性之影響。毒性試驗顯示，將淡水長臂大蝦幼生飼育用水以電解水機電解生成含不同有效氯之電解水，包括含 0 (控制組)、0.2、0.3 及 0.4 mg/L 之有效氯，結果顯示其死亡率分別為 0%、12%、27% 及 42%。而以不同處理方法還原電解水，包括強曝氣 (1 L/min)、活性碳 (50 g) 及控制組 (未處理)，顯示以活性碳吸附有效氯效果最好，10 mg/L 有效氯經活性碳吸附 10 分鐘後，降至約為 1.7 mg/L，且經 60 分鐘後濃度趨近於 0 mg/L。為瞭解活性碳處理後之電解水是否仍具毒性，使用活性碳處理 (50 g) 有效氯濃度為 0 mg/L (控制組)、0.5 mg/L、1 mg/L 及 2 mg/L 之電解水中，並於不同時間點觀察其死亡情況，結果顯示經過 60 小時後，蝦苗皆無死亡的情形發生。根據上述實驗所得最適電解水機使用條件結果，實際應用於淡水長臂大蝦蝦苗培育顯示，電解水應用於淡水長臂大蝦孵化育苗，能有效降低總生菌數及含氮化合物，且電解水組之蝦苗發育較快，活存率亦較佳。電解水組經 28 天後即發現後期蝦苗，且經 37 天後有近九成蝦苗變態為後期蝦苗；另外活性碳組經 31 天後即發現後期蝦苗，且經 40 天後有近九成蝦苗變態為後期蝦苗。實驗結束後，電解水組之蝦苗育成率明顯較活性碳組佳，其育成率分別為 7.6% 及 2.9%。推測其原因為水質改善，進而使蝦苗相對較健康。

Parallel Abstract 〈TOP〉

Student ID: M9813012
Title of Thesis: Usage of electrolytic water system in giant freshwater prawn 
hatchery 
Total Page: 88
Name of Institute: Department of Aquaculture, National Pingtung      
University of Science and Technology
Graduate date: June 20, 2011                Degree conferred: Master
Name of Student: Li Fan Sie                Adviser: Dr. Chun Hung Liu
Dr. Shinn Pyng Yeh
The Contents of Abstract in This Thesis:
   The purpose of this study is to use electrolytic water system in the giant freshwater prawn (Macrobrachium rosenbergii) hatchery while analyze its feasibility application on disease control and water quality improvement. In order to understand the relationships among salinities、flow rates、electric power and effectiveness in production of available chlorine, this study will include different salinities (15 and 30‰), flow rates (3 and 6 L/min), and electric powers (1 and 5 W). The results showed that the available chlorine in electrolytic water was increased as the salinitly and electric power increased. However, the available chlorine production was negative related to flow rate. The experiment showed that the growth of six important aquatic pathogens were well inhibited by incubation with 0.5 mg/L and 1 mg/L of available chlorine in electrolytic water for 30 and 60 seconds, and the 1 mg/L of available chlorine has batter bactericidal effect in Vibrio alginolyticus (host Taiwan abalone), V. alginolyticus (host White shrimp), Vibrio vulnificus (host White shrimp), Photobaterium damsela subsp. damselae (host Tiger shrimp), Streptococcus sp. (host Giant grouper), and Vibrio parahemolyticus (host Taiwan abalone) than that of 0.5 mg/L. In addition, the removal of nitrogen compounds by electrolysis at different salinities (15 and 30‰) and fixed electric power at 5 W have significant effects. The electrolytic water could effectively remove the ammonia-N (NH3-N) and nitrite-N (NO2-N) in which the concentration of NH3-N and NO2-N reached 0 mg/L after 30 min and 10 min, respectively, of reaction with electrolytic water. In order to know more about the possibilities application of electrolytic water on the giant freshwater prawn hatchery, the toxicity test was done. The trial included different concentrations of available chlorine, 0, 0.2, 0.3, and 0.4 mg/L, in which larvae had the mortality of 0%, 12%, 27%, and 42%, respectively after 72 hours, Three types of method on reducing the residue of available chlorine in the electrolytic water including control (without treatment), vigorous aeration (1 L/min), and filtration through activated carbon (50 g) were used in this trial. The best approach for reducing the availabe chlorine is activated carbon which reduces the available chlorine from 10 mg/L down to 1.7 mg/L after 10 min. In order to make sure the electrolytic water was safe for larvae after filtered through activated carbon (50 g), prawn larvae were reared in activated carbon treated electrolytic water, which contain the available chlorine at initial concentration of 0, 0.5, 1, and 2 mg/L, and no dead larvae was observed within 60 hours. Base on the results from the preliminary experiments, the on farm usage of electrolytic water system in giant freshwater prawn hatchery could effectively reduce the total bacteria count, nitrogen compounds, and increased the growth and survival rate of prawn larvae. The prawn in electrolytic water group developed faster than in activated carbon group in which post-larvae was first seen at 28 day, and > 90% of larvae reached post-larvae at 31 day, while the carbon group had the first post-larvae at 31 day, and > 90% of them metamorphosed to post-larvae at 40 day. At the end of experiment, the survival rate of prawn in electrolytic water group was significantly better than that of the carbon group, in which the former is at 7.6% and the latter is 2.9%. These results had make us to believe that the electrolytic water group has excellent water quality and lower bacterial count resulting in accelerated larval development and increased survival rate.

Reference ( 217 ) 〈TOP〉

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Times Cited (1) 〈TOP〉

陳怡樺(2016)。低動力循環水系統對淡水長臂大蝦池水質、成長及活存率之影響。臺灣大學漁業科學研究所學位論文。2016。1-61。

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