以MALDI-TOF質譜分析牛樟芝中之生物性成分

郭朝正

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以MALDI-TOF質譜分析牛樟芝中之生物性成分

Analysis of Bioactive Components by MALDI-TOF MS in Antrodia cinnamomea

郭朝正 , Masters　　Advisor：曾耀銘;劉炳嵐　　

繁體中文

金奈米粒子； MALDI-TOF MS ；醌類衍生物；三萜類；牛樟芝； Ubiquinone derivatives ； Triterpenoids ； Antrodia cinnamomea ； MALDI-TOF MS ； Gold nanoparticles

Abstract │ Reference (82) │ Altmetrics

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Antrodia cinnamomea (AC) is a unique mushroom of Taiwam. Triterpenoids and ubiquinone derivatives possess a variety of biological activities such as anticancer, antimicrobial, anti-inflammatory, antioxidant, and hepatoprotive. In the present study, citrate-capped gold nanoparticles (AuNPs) was utilized as a matrix for the analysis of triterpenoids and ubiquinone derivatives by MALDI-TOF/MS. AuNPs are deposited onto the top of the sample layer, resulting in a increase of the signal intensities of the protonated ions and reproducibility. The method has been successfully employed to measured 9 triterpenoids and ubiquinone derivatives in A. cinnamomea. The identified analytes include methyl antcinate B (AC1), antcin B (AC5), Dehydrosulphurenic acid (AC6), antcin H (AC7), antcin A (AC9), antcin C (AC10), methyl antcinate A (AC11), antcin K (AC12), methyl antcinate H (AC13), and 4-acetylantroquinonol B (4AAQB). The results, showing the linear range and r2 value are 1 ppm~20 ppm with r2 = 0.957 ~ 0.985, respective. This method can fast distinguish the different sources of A. Cinnamomea. Comparison bioactive components between fruiting body and mycelium.

Reference ( 82 ) 〈TOP〉

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連結：
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連結：
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連結：
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連結：
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