以MALDI-TOF質譜分析牛樟芝中之生物性成分

郭朝正

3.238.70.175，Hello！

Close
Search Quick Search Advanced Search Publication Search	Personal Service Member Login Searching History Use Bonus Card SYMSKAN
Browse Browse by University of Graduate Thesis Browse by Pinyin of Graduate Thesis Browse by Conference Proceedings Topic Browse by Conference Proceedings Publisher Browse by Pinyin of Conference Proceedings Browse by eBook Category Browse by eBook Topic Browse by eBook Publisher
Customer Services Authorize Airiti Recommend a Journal

Search Symbol (Half-width)	Description of Search Symbols
Space	"AND" indicates the intertwining of key terms used in a search
Double Quotation Marks ("") ( " " )	Double quotation marks indicate the beginning and end of a phrase, and the search will only include terms that appear in the same order of those within the quotations. Example: "image process" ： " image process "
?	Indicates a variable letter. Entering two ? will indicate two variable letters, and so on. Example: "Appl?", search results will yield apple, apply… e , appl y … ( (often used to English word searches) )
*	Indicates an unlimited number of variable letters to follow, from 1~n. Example: Enter "appl*", search results will yield apple, apples, apply, applied, application…(often used in English word searches) e , appl es , appl y , appl ied , appl ication … ( (often used to English word searches) )
AND、OR、NOT	Boolean logic combinations of key words is a skill used to expand or refine search parameters. (1) AND (1) AND: Refines search parameters (2) OR (2) OR: Expands search parameters (3) NOT: Excludes irrelevant parameters

DOI stands for Digital Object Identifier （ D igital O bject I dentifier ） ,
and is the unique identifier for objects on the internet. It can be used to create persistent link and to cite articles.

Using DOI as a persistent link

To create a persistent link, add「http://dx.doi.org/」「 http://dx.doi.org/ 」 before a DOI.
For instance, if the DOI of an article is 10.5297/ser.1201.002 , you can link persistently to the article by entering the following link in your browser: http://dx.doi.org/ 10.5297/ser.1201.002 。
The DOI link will always direct you to the most updated article page no matter how the publisher changes the document's position, avoiding errors when engaging in important research.

Cite a document with DOI

When citing references, you should also cite the DOI if the article has one. If your citation guideline does not include DOIs, you may cite the DOI link.

DOIs allow accurate citations, improve academic contents connections, and allow users to gain better experience across different platforms. Currently, there are more than 70 million DOIs registered for academic contents. If you want to understand more about DOI, please visit airiti DOI Registration （ doi.airiti.com ）。

Data Source

Basic & Applied Sciences > Chemistry
College of Science and Engineering > Master & Ph.D. Program, Department of Applied Chemistry
Bio-Agriculture > Biological Science

Bibliography Management Tool

Related Links

Report an Issue

Search in Library

There is no full-text available at the moment. Airiti would like to request author's authorization. I would like to provide authorization.

以MALDI-TOF質譜分析牛樟芝中之生物性成分

Analysis of Bioactive Components by MALDI-TOF MS in Antrodia cinnamomea

郭朝正 , Masters　　Advisor：曾耀銘;劉炳嵐　　

繁體中文

金奈米粒子； MALDI-TOF MS ；醌類衍生物；三萜類；牛樟芝； Ubiquinone derivatives ； Triterpenoids ； Antrodia cinnamomea ； MALDI-TOF MS ； Gold nanoparticles

Abstract │ Reference (82) │ Altmetrics

Abstract 〈TOP〉

Parallel Abstract 〈TOP〉

Antrodia cinnamomea (AC) is a unique mushroom of Taiwam. Triterpenoids and ubiquinone derivatives possess a variety of biological activities such as anticancer, antimicrobial, anti-inflammatory, antioxidant, and hepatoprotive. In the present study, citrate-capped gold nanoparticles (AuNPs) was utilized as a matrix for the analysis of triterpenoids and ubiquinone derivatives by MALDI-TOF/MS. AuNPs are deposited onto the top of the sample layer, resulting in a increase of the signal intensities of the protonated ions and reproducibility. The method has been successfully employed to measured 9 triterpenoids and ubiquinone derivatives in A. cinnamomea. The identified analytes include methyl antcinate B (AC1), antcin B (AC5), Dehydrosulphurenic acid (AC6), antcin H (AC7), antcin A (AC9), antcin C (AC10), methyl antcinate A (AC11), antcin K (AC12), methyl antcinate H (AC13), and 4-acetylantroquinonol B (4AAQB). The results, showing the linear range and r2 value are 1 ppm~20 ppm with r2 = 0.957 ~ 0.985, respective. This method can fast distinguish the different sources of A. Cinnamomea. Comparison bioactive components between fruiting body and mycelium.

Reference ( 82 ) 〈TOP〉

1. Asara, J. M., and Allison, J., “Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive,” Analytical Chemistry, 71, 2866-2870 (1999).
連結：
2. Bahr, U., Karas, M., and Hillenkamp, F., “Analysis of biopolymers by matrix-assisted laser desorption/ionization mass spectrometry,” Fresenius Journal of Analytical Chemistry, 348, 783-791 (1994).
連結：
3. Chang, T. T., and Chou, W. N., “Antrodia cinnamomea reconsidered and A.salmonea sp. nov. on Cunninghamia konishii in Taiwan,” Botanical Bulletin of Academia Sinica, 45, 347-52 (2004).
連結：
4. Chang, T. T., and Chou, W. N., “Antrodia cinnamomea sp. nov. on Cinnamomea kanehirai in Taiwan,” Mycological Research, 99, 756-758 (1995).
連結：
5. Chen, Y. T., Hsu, C.L., Hou, S. Y., “Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleases,” Analytical Biochemistry. 375, 299-305 (2008).
連結：

Altmetrics

〈TOP〉

E-mail ：

When an article is available to download, a notice will be sent to your mailbox address.

E-mail ：