工程塑膠PEEK以電漿表面改質及接枝聚合固定肝素之研究

Immobilization of Heparin on Engineering Plastic PEEK by Plasma Treatment and Graft Process

陳欣蘋(Shin-Ping Chen)

指導教授 ： 陳克紹

大同大學/工程學院/材料工程學系所/碩士(2007年)

摘要

本研究以PEEK(polyetheretherketone，聚醚醚酮)為基材使用六甲基二矽胺烷(Hexamethyldisilazane，HMDSZ)單體以電漿輔助化學沉積法 (PECVD) 沉積一層與材料附著性良好的薄膜於PEEK上並利用UV 光誘導接枝聚合丙烯醯胺(Acrylamide, AAm)單體，使材料表面形成特殊官能基，再進ㄧ步利用交聯反應將肝素固定於表面上，進而改善材料生物適應性。因薄膜很薄不容易量測確認，故同時也用壓電石英晶體震盪器(QCM，quartz crystal microbalance)來評估。結果顯示PEEK經HMDSZ電漿沉積處理後，其表面變為疏水性，為一表面形貌均勻之薄膜。固定肝素後水接觸角下降至29°，且其表面粗糙度約為3.8nm。經由Micro-IR表面鍵結改變可知PEEK表面經連續不同處理後，可成功固定肝素。由XPS分析可確認肝素固定之S元素，其原子百分比為2.1％。再經由QCM確認肝素的固定量，其頻率下降量為652Hz。由纖維母細胞培養結果可知PEEK表面固定肝素後可增加其細胞貼附性，具有較佳之生物適應性。

關鍵字

六甲基二矽胺烷 ； 聚醚醚酮 ； 接枝聚合 ； 電漿輔助化學氣相沉積法 ； 肝素 ； 壓電石英晶體震盪器 ； 丙烯醯胺

並列摘要

In this study, the plasma enhanced chemical vapor deposition (PECVD) of hexamethyldisilazane (HMDSZ) was used to obtain a thin adhesive film on the surface of polyetheretherketone (PEEK). The film was subsequently grafted with UV-light induced polymerization of acrylamide (AAm), which can provide useful functional group for immobilizing Heparin to improve biocompatibility. Quartz crystal microbalance (QCM) was used to measure the effect of PEEK surface modification, because the film thickness is too thin to be easily estimated. After plasma deposition of HMDSZ film, the surface of PEEK exhibited hydrophobic feature. After heparin was immobilized as the outer layer of PEEK surface, the measured water contact angle decreased to 29° and the root mean square surface roughness was determined to be 3.8 nm. From micro-IR spectra, heparin was successfully immobilized on the surface of PEEK. According to X-ray photpelectron spectrometer (XPS) analysis, the element sulfur containing 2.1% atomic concentration revealed that heparin was covalently immobilized on PEEK. Frequency shift of 652 Hz detected by QCM was used to determine the amount of immobilized heparin. From the results of fibroblast cell culture, PEEK immobilized with Heparin can indeed promote cell attachment.

並列關鍵字

PEEK ； PECVD ； Graft ； Heparin ； QCM ； HMDSZ ； AAm

參考文獻

28. D. R. Lloyd and C. M. Burns, Journal of Polymer Science, Part A:

29. D. R. Lloyd and C. M. Burns, Journal of Polymer Science, Part A:

25. B. D. Ranter, A. B. Jonston, and T. J. Lenk, “Biomaterial surface”

Science, Part B: Polymer Physics, 29 (1991), pp.119-128

Macromolecules, 18 (1985), pp.86-93