聚甘???生產菌之篩選及培養條件探討與酵素特性分析

Isolation and cultivation conditions of a mannanase producing microorganism and characterization of enzyme properties

邱秉毅(Biing-Yih Chiou)

指導教授 ： 林澤群

中臺科技大學/健康科學院/食品科技研究所/碩士(2008年)

https://doi.org/10.6822/CTUST.2008.00010

摘要

本研究以中部地區土壤篩選出一株可分泌高單位聚甘露糖?活性之菌株。經鏡檢觀察其特徵及比對其18S rDNA 序列後，暫命為 Aspergillus niger NCH-318。Asp. niger NCH-318 所生產之聚甘露糖?粗酵素特性如下：最適反應 pH 4.0，最適反應溫度為60℃，最適反應基質濃度為0.9％。Asp. niger NCH-318 較適培養基與培養條件如下：培養基為Mandels-Reese medium（M），碳源為去油椰子粕，添加量為1.5％，氮源為酵母萃取物，添加量為0.56％，培養溫度為40 ℃，起始 pH 為4.0，接種量為106 spores/ml，震盪速率為110 rpm，在培養六天後，可得最高酵素活性，約為190 U/ml。

關鍵字

Aspergillus niger ； 聚甘露醣 ； 葡萄糖甘露聚醣 ； 半乳糖甘露聚醣 ； 聚甘露糖

並列摘要

A high mannanase-producing fungal strain was isolated soil sample in central Taiwan. According to morphology and 18S rDNA comparison by microscope and comparison between the partial sequence of 18S rDNA, it was identified to be Asp. niger and was therefore named temporarily as A.niger NCH-318. The enzyme properties of crude mannanase are described as follows: the optimum pH was pH 4.0 and optimum temperature was 60℃. The optimum substrate concentration was 0.9%. The optimal medium composition and cultivation conditions for A. niger NCH-318 are determined using shaking flasks (capacity 250 ml). The results showed that Mandels-Reese medium (M) with 1.5% coconut meal which was defatted by n-hexane, 0.56% yeast extract as nitrogen source, incubation temperature at 40℃, initial pH at 4.0, inoculum size at 106 sproes/ml and shaking rate at 110 rpm. The final enzme activity of 190 U/ml in the culture broth was obtained after 6 days of cultivation.

並列關鍵字

mannanase ； Asp. niger ； mannan ； glucomannans ； galactomannans

參考文獻

麥揚竣，2007， Aspergillus oryzae NCH-42 單寧?之生產、純化及特性分析。中興大學食品科學系碩士論文，台中市。

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