- 期刊
緩衝區設置對基因轉殖水稻花粉媒介基因流佈之影響
Effect of Buffer Zones on Pollen-Mediated Gene Flow of Transgenic Rice
摘要
本研究利用2個水稻品種，轉殖植酸酵素水稻（Oryza sativa L. ssp. japonica; AAN）作為花粉貢獻親，台農糯73號（O. sativa L. ssp. japonica cv. 'Tainung73'; 'TNGW73'）作為花粉接受親，進行緩衝區設置影響對基因轉殖水稻花粉流佈之田間評估，以瞭解基因轉殖水稻在商業化生產種植前，對目前之水稻耕作系統有無影響。在有無緩衝區設置對水稻花粉流佈之評估試驗中，顯示有無緩衝區設置的效果受到距離及風向所影響。在順風狀況下，位於下風南方與東南方7m之內，有無緩衝區設置對花粉流佈頻率差異不大，然而在9m處 其花粉流佈頻率有顯著的差別。緩衝區設置南方與東南方9m處的花粉流佈頻率分別為0.83％和0.84％，而無緩衝設置則分別為1.53％和1.65％。在逆風狀況下，位於上風北方與東北方1、3和5m處有緩衝區設置的花粉流佈頻率高於無緩衝區設置，但在7m以後有緩衝區設置的花粉流佈頻率則明顯低於無緩衝區設置，顯示在上風處之風速較小的情況下，7m的緩衝區設置可減少花粉流佈頻率。
並列摘要
In this study, the non-glutinous of phytase-transformed rice line AAN (Oryza sativa L. ssp japonica) was used as the pollen donor and the glutinous rice 'Tainung waxy 73' (O. sativa L. ssp. japonica cv. 'TNGW73') was used as the pollen recipient. The objective of this research was to study the effect of buffer zones on pollen dispersal of transgenic rice in the open fields. Results showed that the frequency of outcrossed seeds varied with the distances and directions. The frequencies of outcrossed seeds were not significantly different in the south and southeast directions within 7m between buffer zones and non-buffer zones treatments. However, there were significantly different frequencies of outcrossed seeds at 9-m distance between buffer zones and non-buffer zones treatments. The frequency of outcrossed seeds with buffer zone was 0.83% (south) and 0.84% (southeast) and 1.53% (south) and 1.65% (southeast) for non-buffer zones, respectively. The frequency of outcrossed seeds of plots with buffer zones was higher than those without buffer zones in the north and northeast at distance of 1, 3, and 5m. However, the frequency of outcrossed seeds of plots with buffer zones was significantly lower than those without buffer zone beyond 7m. This study indicated buffer zones longer than 7m could decrease the frequency of outcrossed seeds by pollen-mediated gene flow in the directions against wind.
延伸閱讀
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