次氯酸降解試驗及其在滅菌細本山葡萄莖節培植體之應用

Degradation Test of Hypochlorous Acid and Its Application in the Disinfection of Vitis thunbergii Sieb. & Zucc. Stem Node Explants

蕭翌柱(Yih-Juh Shiau) ； 陸明德(Ming-Te Lu) ； 林宗俊(Tsung-Chun Lin)

《台灣農業研究》 73卷1期 (2024/03) Pp. 25-36

https://doi.org/10.6156/JTAR.202403_73(1).0003

摘要

次氯酸（HOCl）具有強氧化力且能破壞微生物細胞膜通透性達到消毒滅菌的成效，本試驗之主要目的，在於檢測不同光照強度或高溫高壓滅菌後對HOCl濃度降解的影響；此外，探討含不同濃度HOCl溶液外部消毒處理，對於降低細本山葡萄（Vitis thunbergii Sieb. & Zucc.）莖節培植體汙染率的影響。結果顯示，將含HOCl濃度為198.3±3.4mg L^(-1)的溶液盛裝於深褐色血清瓶，並放置在室內、常溫且避免陽光直射的環境，可維持其穩定性達5d以上；另經高溫高壓滅菌後的HOCl含量變化不大。使用0.6%（w/v）次氯酸鈉（NaOCl）溶液進行細本山葡萄莖節外部消毒20min之培植體汙染率為82.2±1.6%（對照組），顯著高於使用150與200mg L^(-1) HOCl溶液消毒的處理組（61.1±5.7% & 57.8±4.2%），且經21 d培養後也未見HOCl影響莖節正常生長。因此，培植體在外部消毒處理時，以HOCl取代NaOCl的構想具可行性。

關鍵字

高壓滅菌 ； 光照度 ； 微生物汙染 ； 組織培養

並列摘要

Hypochlorous acid (HOCl) possesses potent oxidative capabilities and can disrupt the permeability of microbial cell membranes, thereby achieving sterilization. The main purpose of this study was to determine the impact of different light illuminations or high-temperature autoclaved sterilization on the degradation of HOCl. Furthermore, the study explored the effects of external disinfection treatments with different concentrations of HOCl on reducing the contamination rate of Vitis thunbergii Sieb. & Zucc stem node explants. The results showed that the stability of the solution containing 198.3 ± 3.4 mg L^(-1) of HOCl could be maintained for more than 5 d if it was placed in brown glass bottled indoors at room temperature in the shade. Minimal alteration in the HOCl content was observed after autoclaving. When 0.6% (w/v) sodium hypochlorite (NaOCl) solution was used for external disinfection of Vitis thunbergii Sieb. & Zucc stem nodes for 20 min, the explant contamination rate was 82.2 ± 1.6% (Control), which was significantly higher than the treatments using 150 and 200 mg L^(-1) HOCl solutions (61.1 ± 5.7% & 57.8 ± 4.2%). After a 21-day culture period, HOCl demonstrated no adverse effects on the normal growth of stem nodes. Thus, replacing NaOCl with HOCl for external disinfection of explants appears to be a viable alternative.

並列關鍵字

Autoclaving ； Light illumination ； Microbial contamination ； Tissue culture