- 期刊
次氯酸水對蝴蝶蘭軟腐病防治效果評估
Evaluation of Control Efficacy of Hypochlorous Acid Against Phalaenopsis Soft Rot
摘要
蝴蝶蘭為臺灣重要外銷花卉，年產值將近46億元。然而蝴蝶蘭細菌性軟腐病（bacterial soft rot）是影響臺灣蝴蝶蘭栽培期與外銷產值的重要病害之一。本研究利用次氯酸水（hypochlorous acid; HOCl）溶液作為蝴蝶蘭軟腐病防治資材，進行防治效果試驗評估。首先利用不同接種濃度的軟腐病菌測試對蝴蝶蘭軟腐病發生的影響，由結果得知，高濃度菌液10^8 CFU mL^(-1)接種後，其發病率為58.3－81.3%，10^6 CFU mL^(-1)之發病率25.0－43.8%，但低濃度10^4 CFU mL^(-1)其發病率降至為0，隨著軟腐病菌接種濃度降低，蝴蝶蘭葉片感染軟腐病之發病率逐漸下降。室內試驗結果所示，以HOCl溶液10 mg L^(-1)處理軟腐病菌（10^8 CFU mg L^(-1)）10 min可完全抑制供試菌株之生長。盆栽試驗（接種濃度10^8 CFU mL^(-1)）結果得知，20 mg L^(-1) HOCl濃度下，對蝴蝶蘭軟腐病具有18.0－66.7%防治率，當濃度提高至50 mg L^(-1)下，防治率則可達50.0－88.9%，與對照組56.3－88.7%之發病率有顯著性差異，顯示HOCl溶液可降低軟腐病的發病率。因此，本研究之結果得知，HOCl溶液可作為田間防治蝴蝶蘭軟腐病之參考。
並列摘要
Phalaenopsis orchids are important export flowers in Taiwan, with an annual production value of nearly 4.6 billion NT dollars. However, bacterial soft rot significantly affects their cultivation and export value. Therefore, this study evaluates the effectiveness of hypochlorous acid (HOCl) solution as a control material for preventing bacterial soft rot in Phalaenopsis orchids. Different inoculum concentrations of the rot bacteria were tested to assess their impact on disease occurrence. Results showed that at a high concentration of 10^8 CFU mL^(-1), the disease incidence was as high as 58.3-81.3%, while at 10^6 CFU mL^(-1), it was 25.0-43.8%. At a low concentration of 10^4 CFU mL^(-1), the incidence decreased to 0%, indicating a gradual reduction in disease occurrence with lower bacterial concentrations. Laboratory experiments demonstrated that treating the bacteria (10^8 CFU mL^(-1)) with a 10 mg L^(-1) HOCl solution for 10 min inhibited their growth. Pot experiments revealed that at a HOCl concentration of 20 mg L^(-1), the control efficacy against bacterial soft rot ranged from 18.0% to 66.7%. In contrast, the control efficacy increased from 50.0% to 88.9% at 50 mg L^(-1), showing significant differences in control effectiveness. Thus, HOCl solution can control the bacterial disease of Phalaenopsis soft rot caused by Dickeya fangzhongdai in orchids.