Biological Activities of Essential Oil Obtained from Cymbopogon citratus on Crithidia deanei

Raíssa Bocchi Pedroso ； Tânia Ueda-Nakamura ； Benedito Prado Dias Filho ； Diógenes Aparício Garcia Cortez ； Lúcia Elaine Ranieri Cortez ； José Andrés Morgado-Díaz ； Celso Vataru Nakamura

《Acta Protozoologica》 45卷3期 (2006/09) Pp. 231-240

並列摘要

We report the effect of the essential oil extracted from Cymbopogon citratus on endosymbiont-harbouring and endosymbiont-free strains of the insect trypanosomatid Crithidia deanei grown at 28℃ in a chemically defined medium. A dose-dependent antiprotozoan effect of the essential oil of C. citratus could be observed on both strains of C. deanei. The IC50 values (50% inhibitory concentration) for symbiont-bearing and symbiont-free strains were 120 and 60 μg/ml, respectively. The viability assay showed that the symbiont-free strain is more sensitive to the presence of the essential oil, because lysed cells were observed after 2 h of exposure at higher concentrations. In addition, alterations in the ultrastructure and in the detection of cell-surface carbohydrate residues in both strains of C. deanei after treatment with the essential oil were also evaluated. Both strains showed ultrastructural alterations in the cellular and flagellar pocket membranes, as revealed by transmission electron microscopy. In the lectin assay, the essential oil influenced the expression of carbohydrates in symbiont-free C. deanei, as evidenced by a reduction of sialic acid residues on the cell surface.

並列關鍵字

antiprotozoan activity ； Crithidia deanei ； Cymbopogon citratus ； medicinal plants