乳品、禽肉、豬肝中林可黴素(Lincomycin)及健牠黴素(Gentamicin)殘留之檢驗方法探討

Method for Detection Lincomycin and Gentamicin Residues in Milk, Chicken and Suine-Liver

王鳳英(Fung-Ing Wang) ； 張圓笙(Yuarg-Sheng Cheang)

《藥物食品檢驗局調查研究年報》 9號 (1991/09) Pp. 333-340

https://doi.org/10.6946/ASRNL.199109.0333

摘要

本報告係以0.1MpH8.0磷酸液衝液及系統性的溶媒抽取法作選擇性抽取，將各抽取液點在TLC板上，經展開後，再以已接菌種之方型培養基板與之接觸，培養後視所形成抑制圈之Rf值作為鑑別殘留抗生素種類之依據；同時將檢液以圓筒平板法作生物檢定，依據抑制圈之大小來定量。將林可徵素及使牠黴素添加於食品後分別以溶媒或衝液抽取，以圓筒平板法檢測其限量分別如下：林可黴素：0.1~0.5μg/g（依不同或抽取液而不同，健牠黴素0.1μg/g，林可黴素以溶媒和緩衝溶液抽取之平約回收率分別為54.5~77.5%及28.5~81.7%。使牠黴素以溶媒和緩衝溶液抽取之平均回收率則分別在56~83.7%及46.5~52.5%，兩種抗生素之回收率皆以溶媒抽取者較高。生物自析法是以纖維薄層鋁片經7%氯化銨和0.5MpH7.0磷酸緩衝液展開較佳，各種抗生素可依不同之Rf值區分開來。林可黴素及健牠黴素之最低感度分別為0.3及0.05μg。

關鍵字

林可黴素 ； 健牠黴素 ； TLC-生物自析法

並列摘要

A microbiological procedure was evaluated to detect gentamicin and lincomycin residues in food. Antibiotic residues were extracted from samples with methanol and methanol-HCL (98+2). The methanol extract was further extracted with chloroform to isolate a group of antibiotics. The extracts were spotted onto TLC plates and developed by suitable solvent systems. Developed plates were then placed on set medium seeded with Bacillus subtilis. A bioautograph was formed after incubated at 37℃ for 17 hours.
Recoveries of lincomycin added to milk, chicken and swine liver ranged at 4.5-77.5% and 28.5-83.7% with solventsystems and buffer extraction respectively. while recoveries of gentamicin were 56-84.7% with solvent system and 46.5%-52.5% with buffer extraction. The lowest detectable doseof lincomycin and gentamicin with solvent system and phosphate buffer extraction were 0.1-0.5 and 0.1μg/g respectivly.
The TLC-bioautographic method with cellulose aluminum sheet by7% NH4 C1 or pH7, 0.5M phosphate buffer were found to be the best developing system for these two antibiotics. The lowest detectable dose of this method was 0.3μg lincomycin and 0.05μg gentamicin.

並列關鍵字

Lincomycin ； Gentamicin ； TLC-bioautography