食品中四環素類抗生素殘留之檢驗方法探討

Microbiological Method of Test for Tetracyclines Risidues in Foods

張翠瑛(Tsui-Yin Chang) ； 朱淑儀(Shu-Yi Chu) ； 王貞懿(Jen-Yi Wang) ； 張圓笙(Yuarn-Sheng Chang) ； 洪其璧(Chi-Byi Horng)

《藥物食品檢驗局調查研究年報》 9號 (1991/09) Pp. 341-348

https://doi.org/10.6946/ASRNL.199109.0341

摘要

本報告以圓筒平板法及TLC－生物自析法進行豬肉、豬肝、雞肉、雞肝、鮮乳及鮮蛋中四環素類抗生素錢留之檢驗方法探討。
檢體經均質後以0.1%檸檬酸：乙醇(7:3)混合液萃取，再經XAD-2吸附性樹脂管柱淨化，最後以甲醇溶離，所得檢液分別利用圓筒平板法及生物自析法定性定量。圓筒平板法之最低檢出限量輕四環素(OTC)，四環素(TC)均為0.05μg/g，氯四環素(CTC)則為0.01μg/g。鮮肉及內臟中OTC回收率89.7%~92.7%，TC81.3%~84.7%，CTC83.3%~88.0%；鮮乳之CTC平均回收率為86.3%，鮮蛋之CTC平均回收率為73.3%。在生物自析法中以正丁醇：醋酸：去離子水(10:1:2)展開液展開後，Rf分別為CTC0.52, CTC0.32及TC0.43，感度三種均達5ng。
於檢體中分別添加鏈黴素、林可黴素、健牠黴素、新黴素5μg/g，結果均與抑制圈形成；泰黴素、紅黴素在5μg/g時有抑制圈，2.5μg/g時則無抑制圈形成；配尼西林在0.03, 0.02μg/g時有抑制圈，0.01μg/g時則無抑制圈形成。
由市面抽購120件檢體進行實驗，結果僅檢出豬肝1件中有OTC0.062ppm之殘留，符合規定。

關鍵字

食品 ； 四環素類抗生素 ； 殘留 ； 微生物方法

並列摘要

The cup-plate-method and TLC-bioautographic method were tested for analyzing the residual tetracyclines (TCs) in pork, pork liver, chicken muscle, chicken liver, egg and milk.
After extraction with 0.1% citric acid: ethanel solvent (7:3) from homogenized samples the extract was concentrated. The concentrated solution was passed through AmbeiteAD-2 resin column and then eluted with methanol. The eluate was devided into two parts for quantity and qualitative analysis using the cup- plate-method and TLC-bioautography.
The recoveries of OTC added to meats and livers were 89.7% and 927%, respectively; those of TC were 81.3% and 84.7%; those CTC were 83.3% and 88.0%. The average recoveries of CTC add to milk was 88.3%, while CTC in egg was 73.3%. As little as 0.05μg/g(OTC, TC) or 0.01μg/g (CTC) was detectable in cup-plate-method. TLC plate was developed by the solvent system of butanol: acetic acid: deionized water (10:12), their Rf valueswere CTC 0.52, OTC 0.32 and TC 0.43, respectively. All of the sensitivity was 5ng.
Other antibiotics were also added to sample and detected by the cup-plate-method. Results of milk and Streptomycin (5μg/g), Lincomycin (5μg/g), Gentamycin (5μg/g), Neomycin (5μg/g), Tylosin (2.5μg/g), Erythromycine (0.5μg/g) or Penicillin (0.01μ/g) showed no inhibition zone; but in Tylosin (5μg/g), Erythromycin (5μg/g) and penicillin (0.03μ/g, 0.02μ/g) inhibition zones occured.

並列關鍵字

Food ； Tetracyclines ； Residues ； Microbiological Method