- 期刊
Determination of Polar Constituents of Scrophulariae Radix in Bai-He-Gu-Jin-Tang by Micellar Electrokinetic Capillary Chromatography
並列摘要
A micellar electrokinetic capillary chromatographic method was established for the determination of 2-(3-hydroxy-4-methoxyphenyl)ethyl 1-O-[α-L-arabinopyranosyl(1→6)]-feruloyl(1→4)-α-L-rhamnopyranosyl(1→3)-β-D-glucopyra noside (SN-A), harpagoside (SN-B) and cinnamic acid (SN-C) in the traditional Chinese medicinal prescription, Bai-He-Gu-Jin-Tang, which contains Scrophulariae Radix. This chromatographic technique was carried out using a buffer composed of 20 mM sodium tetraborate and sodium dihydrogen phosphate containing 120 mM sodium cholate and 12% methanol at pH 7.5. 2-(4-Hy-droxyphenyl)-ethylammonium chloride was used as an internal standard and detected at 280 nm. The linear calibration ranges for SN-A, B and C were 11.76-188.16 μg/mL (r=0.9998), 8.16-130.56μg/mL (r=0.9992) and 4.48-71.68μg/mL (r=0.9991), respectively. The recoveries of the markers listed above were SN-A: 104.3±2.8%, SN-B: 98.1±2.2% and SN-C: 98.2±1.4%. The relative standard deviations of the three marker substances for intraday and interday analyses ranged from 0.85 to 2.87 % and from 1.42 to 4.14 %, respectively.
延伸閱讀
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