Identification of an Autoantibody against the Proliferating Cell Nuclear Antigen from a Patient with Systemic Lupus Erythematosus

Antibodies against the proliferating cell nuclear antigen (PCNA) as first discovered in the sera of systemic lupus erythematosus (SLE) patients. However, the reactivity and specificity of anti-PCNA autoantibodies are still unclear. To investigate the property of anti-PCNA autoantibodies, conducted an ELISA screening of the anti-PCNA autoantibodies in sera of SLE patients. Eighteen out of 191 SLE sera ere found to be positive for anti-PCNA antibodies giving a frequency of nearly 10%. Among the positive sera, a sample with the highest titer of anti-PCNA autoantibody preferentially recognizes the wild-type PCNA as compared to the Y114A mutation which contains a single amino acid substitution at 114 and fails to form the toroidal structure. Moreover, the autoantibody purified from this serum identifies only the free PCNA in crude mammalian cell extracts but not other associated cellular components. This finding raises a possibility that immunostaining with the human anti-PCNA autoantibodies in previous studies might have only partially PCNAs in tissues.

並列關鍵字

autoantibodies ； systemic lupus erythematosus SLE ； proliferating cell nuclear antigen PCNA ； toroidal structure

參考文獻

Alan, J.,Robin, T.(1987).Precipitation Techniques in Agar and Agarose.(Immunochemistry in Practice).

Google Scholar

Bernstein, R.,Bunn, C.,Hughes, G.,Francoeur, A.,Mathews, M.(1984).Cellular proteins and RNA antigens in autoimmune disease.Mol. Biol. Med..2,105-120.

Google Scholar

Brand, S.,Bernstein, R.,Mathews, M.(1994).Autoreactive epitope profiles of the proliferating cell nuclear antigen define two classes of autoantibodies.J. Immunol..153,3070-3078.

Google Scholar

Bravo, R.,Bravo, H. M.(1987).Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.J. Cell. Biol..105,1549-1554.

Google Scholar

Celis, J. E.,Celis, A.(1985).Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase.Proc. Natl. Acad. Sci. U.S.A..82,3262-3266.

Google Scholar

延伸閱讀

Sung, W. Y., Chen, P. P., Tseng, C. C., Wu, C. C., Ou, T. T., Tsai, W. C., & Yen, J. H. (2014). Anti-SmD and Anti-Sm Antibodies in the Diagnosis of Systemic Lupus Erythematosus. Formosan Journal of Rheumatology, 28(2), 11-16. https://doi.org/10.6313/FJR.2014.28(02).03
梁育瑞（2017）。Evaluation of the effect of monoclonal antibody KIM-1 in differentiating between postmortem autolysis and acute tubular necrosis〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201701571
Kuo, S. F., Chung, C. Y., Lin, J. S., Wang, B. T., & Chang, C. S. (2002). EDTA-dependent Pseudothrombocytopenia in a Patient with Systemic Lupus Erythematosus. The Changhua Journal of Medicine, 7(3), 168-172. https://www.airitilibrary.com/Article/Detail?DocID=16801504-200207-7-3-168-172-a
Shobaili, H. A. A., Robaee, A. A. A., Alzolibani, A., Khan, M. I., & Rasheed, Z. (2011). Hydroxyl Radical Modification of Immunoglobulin G Generated Cross-Reactive Antibodies: Its Potential Role in Systemic Lupus Erythematosus. Clinical Medicine Insights: Arthritis and Musculoskeletal Disorders, (2011), 11-19. https://doi.org/10.4137/CMAMD.S6793
李易達（2016）。Preparation of A Monoclonal Antibody that Recognizes Hepp1〔碩士論文，國立中正大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0033-2110201614043622

國際替代計量

Identification of an Autoantibody against the Proliferating Cell Nuclear Antigen from a Patient with Systemic Lupus Erythematosus

全文下載

主題瀏覽