Immunofluorescent Localization of 24 kDa Protein in Luffa cylindrica Phloem Exudate

絲瓜韌皮部蛋白質的免疫螢光定位

張奕元(Yih-Yuan Chang) ； 陳榮銳(Yung-Reui Chen)

《TAIWANIA》 41卷3期 (1996/09) Pp. 244-251

https://doi.org/10.6165/tai.1996.41.244

摘要

絲瓜韌皮部溢流物經電泳分析及高效能液態色層分析，取得24 kDA的韌皮部蛋白質。此蛋百質打入兔子產生多元的抗體，能認出原性蛋白質或其變性蛋白質次體。以FITC免疫螢光細胞定位法檢出該蛋白質存在位置時，觀察到絲瓜果實內的維管束是其主要存在位置，尤以其雙側型的內生及外生韌皮部最為顯著。類似結果也在枝條及葉柄的維管束表現。

關鍵字

韌皮部蛋白質 ； 免疫螢光 ； 定位 ； 絲瓜

並列摘要

A 24 kDa phloem protein was isolated from phloem exudate of Luffa cylindrica fruits by the method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Polyclonal antibodies obtained from rabbit serum recognized both the native protein and denatured protein subunits. Immunofluorescent study was done by a FITC-conjugated goat-anti-rabbit IgG. Immunolabelled sections of pepo show that the antibodies strongly bound with 24 kDa protein in the bicollateral phloem tissues of vascular bundle. The intensity of green fluorescence is related to the degree of phloem differentiation. Similar results were also observed in stems and petioles of leaves.

並列關鍵字

Phloem protein ； Immunofluorescence ； Localization ； Luffa cylindrica