Water spinach (Ipomoea Aquatic Forsk) is an important tropical vegetable in Taiwan. The temperature has great impact on its production yield as well as quality. Due to climate change, recent severe cold air mass had caused its production reduction. Therefore, it is vital to assess the effect of temperature and time on causing chilling injury of I. aquatic Forsk. The research results showed that 12℃was the critical temperature to cause the chilling injury of the I. aquatic Forsk. The high ratio of chilling injured leaves, which affected the commercial values, was occurred at 12℃ treated for 12hr, 10℃ treated for 6hr, and 6℃ treated for 4hr. The ratios were 60%, 42.5%, and 31.6% respectively. This result is useful not only to establish the low temperature warning system of I. aquatic but also to help the research of chilling injury of I. aquatic for prevention.