- 期刊
Expression of Gastrin and Its Relationship to Cell Proliferation and Oncogene Expression in Colorectal Cancer
大腸直腸癌組織胃泌素與細胞增殖及癌基因表現之關係
摘要
Objectives: To investigate the expression of gastrin and its relationship to the proliferative status and oncogene expression in colorectal tissues, and to explore their clinical significance. Methods: The expression of gastrin, c-myc, c-fos, rasP2l and PCNA in cancer tissue and cancer-adjacent mucosa (CAM, 3cm and 6cm, respectively) from 48 cases of colorectal cancer (CRC) and in normal mucosa from 10 control subjects were investigated by immunohistochemistry techniques. Argyrophilia nucleolar organizer regions (AgNORs) were determined by argyrophilia stain. Results: The positive expression of gastrin in CRC tissue was 39.6%, obviously higher than in CAM 3cm (4.2%), CAM 6cm (0%) and normal mucosas (0%). The positive rate of gastrin in well-differentiated adenocarcinoma was higher than that in poorly differentiated carcinoma and mucinous adenocarcinoma (P＜0.05). The PCNA-LI, AgNORs count and the positive rates of c-myc, c-fos and rasP2l in CRC were obviously higher than those in CAM and normal mucosas (P＜0.01). In the group of gastrin-positive expression, the PCNA-LI, AgNORs count and the positive rates of c-myc, c-fos were significantly higher than those in the group of gastrin-negative expression (P＜0.05). Conclusions: Some colorectal cancer cells can produce gastrin, especially in well-differentiated adenocarcinoma. Endogenous gastrin might increase the oncogene expression of c-myc, c-fos etc and trigger the colorectal cancer cell proliferation. Gastrin may be a potential therapeutic target in the treatment of colorectal cancer.
關鍵字
無資料並列摘要
Objectives: To investigate the expression of gastrin and its relationship to the proliferative status and oncogene expression in colorectal tissues, and to explore their clinical significance. Methods: The expression of gastrin, c-myc, c-fos, rasP2l and PCNA in cancer tissue and cancer-adjacent mucosa (CAM, 3cm and 6cm, respectively) from 48 cases of colorectal cancer (CRC) and in normal mucosa from 10 control subjects were investigated by immunohistochemistry techniques. Argyrophilia nucleolar organizer regions (AgNORs) were determined by argyrophilia stain. Results: The positive expression of gastrin in CRC tissue was 39.6%, obviously higher than in CAM 3cm (4.2%), CAM 6cm (0%) and normal mucosas (0%). The positive rate of gastrin in well-differentiated adenocarcinoma was higher than that in poorly differentiated carcinoma and mucinous adenocarcinoma (P＜0.05). The PCNA-LI, AgNORs count and the positive rates of c-myc, c-fos and rasP2l in CRC were obviously higher than those in CAM and normal mucosas (P＜0.01). In the group of gastrin-positive expression, the PCNA-LI, AgNORs count and the positive rates of c-myc, c-fos were significantly higher than those in the group of gastrin-negative expression (P＜0.05). Conclusions: Some colorectal cancer cells can produce gastrin, especially in well-differentiated adenocarcinoma. Endogenous gastrin might increase the oncogene expression of c-myc, c-fos etc and trigger the colorectal cancer cell proliferation. Gastrin may be a potential therapeutic target in the treatment of colorectal cancer.
延伸閱讀
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