- 期刊
The Role of Long-form Collapsin Response Mediator Protein-1 (LCRMP-1) in Cancer Angiogenesis
並列摘要
Angiogenesis is a physiological process that is essential for development, and is also critical for many diseases such as cancer progression. Until now, the regulation mechanism of tumor angiogenesis is still unclear. Several positive and negative regulators and mechancial signals like small GTPase are found to be involved in the controlling of tumor angiogenesis. Recently, a new member of Collapsin Response Mediator Protein (CRMP) fanily called long-form CRMP-1 (LCRMP-1) has been identified as an invasion enhancer. It promotes cancer cell migration, invasion, and metastasis and even accelerates filopodia formation through binding to the actin nucleation scaffold protein, WAVE-1. In this study, we further investigate the role of CRMP-1 in tumor angiogenesis; dissect the potential molecular actions of LCRMP-1 in the process of neovascularization. Overexpression of LCRMP-1 promotes xenograft tumor growth in vivo and endothelial cell tube formation in vitro. Using immunohistochemical staining, we find that the protein expression of LCRMP-1 correlates with microvessel density in non-small cell lung cancer (NSCLC) patients. Comparing the protein expression profiles of LCRMP-1 overexpression or knocking down stable cells with the control groups, we fond that LCRMP-1 may promote tumor angiogenesis through increasing several angiogenesis factors like serpin E1, coagulation factor etc. In conclusion, LCRMP-1 not only plays as a cancer invasion enhancer but also plays an important role in cancer angiogenesis. These findings indicate that LCRMP-1 uses multiple mechanisms to promote cancer metastasis and may serves as a portential target for the development of cancer treatment.
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