Prefabricated cold stores are commonly used by farmers in Taiwan for pre-cooling and storing their products. In a cold store, the distribution of temperature that could affect qualities of product is influenced by the flow patterns directly. Therefore, it is very important to understand the flow patterns in a cold store. Experimental approaches are used in this study to investigate the air flow characteristics in a prefabricated cold store. A three dimensional ultrasonic anemometer is employed to measure the turbulence characteristics of air flow. Mean airflow patterns and temperature distributions in various sections of the clod store are discussed in detail. The current founding is not only significant for understanding the airflow behavior in cold stories but also important in improving the modeling method for airflow predictions.