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The ETS/IGS Region in a Lower Animal, the Seawhip, Junceella fragilis (Cnidaria: Anthozoa: Octocorallia): Compactness, Low Variation and Apparent Conservation of a Pre-rRNA Processing Signal with Fungi

白蘆莖鞭珊瑚核醣核酸之外轉錄和基因區間:精簡、低變異與蕈類Pre-rRNA處理序列的顯著保守

摘要


我們定序白蘆莖鞭珊瑚核醣核酸之外轉錄和基因區間的核苷酸序列, 發現此區間為一條不典型的短外轉錄和基因區間序列,而且位在外轉錄區間和18S核醣核酸交界處的一組8-bp片段,與蕈類pre-rRNAA1剪切訊息序列呈現出顯著的相似性。這段處理訊息明顯的保守性在其他動物相對的序列中未曾被報導過。白蘆莖鞭珊瑚的核醣核酸基因區間序列缺少一般較高等動物同一序列中的長重複片段,但是在外轉錄區間的3撇端具有一組微衛星序列。比較不同地理區的白蘆莖鞭珊瑚基因區間,顯示不同族群間缺乏明顯的序列變異。

並列摘要


We determined the nucleotide sequence of an atypically short intergenic spacer/external transcribed spacer (IGS/ETS) region in the seawhip, Junceella fragilis, and identified an 8-bp motif at the 5'- ETS/18S rDNA boundary closely resembling a fungal pre-rRNA A1 cleavage signal. This apparent conservation of processing signals was unexpected, as no corresponding sequence has previously been reported in any animal. The J. fragilis IGS sequence lacks repetitive motifs of the type typical of higher animals, but a microsatellite is present in the putative 3'-ETS. Comparisons of the IGS between geographically isolated populations of J. fragilis indicate an unexpected lack of sequence variation.

並列關鍵字

IGS ETS rRNA processing Cnidaria Lower animals

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