利用體外試驗研究探討短期臭氧暴露之揮發性有機物質變化

胡佩儀; 馬一中

doi:10.6288/TJPH2011-30-01-09

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P82 - 94

Health & Medical Care > Health Promotion & Hygiene
Health & Medical Care > Social Medicine

DOI： 10.6288/TJPH

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利用體外試驗研究探討短期臭氧暴露之揮發性有機物質變化

An in vitro Study of short-term Ozone Exposure Induced Volatile Organic Compounds

胡佩儀(Pei-Yei Hu) ；馬一中(Yee-Chung Ma)

台灣公共衛生雜誌； 30卷1期 (2011 / 02 / 15) ， P82 - 94

繁體中文 DOI： 10.6288/TJPH2011-30-01-09

臭氧暴露；人類肺部細胞；揮發性有機物；氣相層析質譜儀； Ozone exposure ； MRC-5 ； VOCs ； GC/MS

Abstract │ Reference (20) │ Altmetrics

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Objectives: The aim of this study was to evaluate the impinger in vitro exposure system used to investigate ozone exposure induced oxidative stress. Comparisons of Volatile Organic Compounds (VOCs) levels in collected gas and MRC-5 cell membrane integrity were made after ozone exposure. In order to validate the source of the VOCs, the reactions of ozone with two polyunsaturated fatty acids (PUFA); Arachidonic Acid (AA) and Linoleic Acid (LA) were also determined. Methods: MRC-5 cells were treated with air and ozone concentrations ranging from 0.1 to 2 ppm for 30 min, and VOCs were collected during and after ozone exposure at 30, 60, 90, and 120 min intervals. PUFAs 10 ppm were exposed to 1 ppm ozone for 30 min. Sampling tubes were used to collect the reacting gas after ozone exposure on human lung cells or PUFAs and these were then analyzed by GC/MS. Results: When the VOCs from air and ozone exposure in MRC-5 cells were compared at different time points, 18 compounds were found to be significantly different. The methyl cyclopentane level increased acutely after oxidative stress to lung tissue. 3-methyl butanal was significantly associated with a high ozone level; carbon disulfide and dimethyl disulfide increased immediately following ozone exposure at 1.5 ppm for 30 min. 2-butanal and methyl cyclopentane were significant different after exposure to ozone 2 ppm as measured by the Tukey HSD test. 2-methyl-1-propene and pentane were also significantly different in the reactive gases of AA and LA after ozone exposure. Conclusions: VOCs may be significant markers of oxidative stress after the human lung cell is exposed to ozone in the impinger exposure system.

Reference ( 20 ) 〈TOP〉

Aufderheide, M.(2008).An efficient approach to study the toxicological effects of complex mixtures.Exp Toxicol Pathol,60,163-80.
Bosson, J.,Pourazar, J.,Forsberg, B.,Adelroth, E.,Sandström, T.,Blomberg, A.(2007).Ozone enhances the airway inflammation initiated by diesel exhaust.Respir Med,101,1140-6.
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Chen, X.,Xu, F.,Wang, Y.(2007).A study of the volatile organic compounds exhaled by lung cancer cells in vitro for breath diagnosis.Cancer,110,835-44.

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