Evaluation of the Use of Different Solvents to Extract the Four Main Components of Yinqiaosan and Their in Vitro Inhibitory Effects on Influenza-A Virus

不同提取溶劑下銀翹散中四種成分指紋圖譜的建立及其體外抗甲型流感病毒效果

王雪峰(Xue-Feng Wang) ； 郝歐美(Ou-Mei Hao) ； 王文麗(Wen-Li Wang) ； 英錫相(Xi-Xiang Ying) ； 王懷良(Huai-Liang Wang)

《The Kaohsiung Journal of Medical Sciences》 26卷4期 (2010/04) Pp. 182-191

摘要

High-performance liquid chromatography coupled with an ultraviolet detector was used to quantitatively determine the amount of caffeoylquinic acid, arctium lappa glycoside, aurantiamarin and forsythin present in five solvent extracts (water, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol) of Yinqiaosan (YQS). The calibration curves of the four components were linear (r^2＞0.998) over the concentration range of 0.9-151.5 μg/mL. This method showed good repeatability with relative standard deviations of less than 2.9% and mean recoveries of 91.3-100.5%. We established the relative amounts of each of the four main ingredients in YQS for all five solvent extracts. The direct inhibitory effects of the five YQS extracts on influenza-A virus in vitro and the therapeutic effects on infected embryonated hen eggs were evaluated. YQS extracted in 50% ethanol and used at concentrations of 500mg/mL and 250mg/mL exerted the greatest therapeutic effect in infected embryonated hen eggs and was significantly superior to ribavirin (p＜0.05). Therefore, the 50% ethanol extract of YQS seems to be the most effective for the inhibition of influenza-A virus. Based on these results, further studies are warranted to evaluate the use of YQS extracts as a possible therapy for influenza.

關鍵字

指紋圖譜 ； A型流感病毒 ； 銀翹散

並列摘要

High-performance liquid chromatography coupled with an ultraviolet detector was used to quantitatively determine the amount of caffeoylquinic acid, arctium lappa glycoside, aurantiamarin and forsythin present in five solvent extracts (water, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol) of Yinqiaosan (YQS). The calibration curves of the four components were linear (r^2＞0.998) over the concentration range of 0.9-151.5 μg/mL. This method showed good repeatability with relative standard deviations of less than 2.9% and mean recoveries of 91.3-100.5%. We established the relative amounts of each of the four main ingredients in YQS for all five solvent extracts. The direct inhibitory effects of the five YQS extracts on influenza-A virus in vitro and the therapeutic effects on infected embryonated hen eggs were evaluated. YQS extracted in 50% ethanol and used at concentrations of 500mg/mL and 250mg/mL exerted the greatest therapeutic effect in infected embryonated hen eggs and was significantly superior to ribavirin (p＜0.05). Therefore, the 50% ethanol extract of YQS seems to be the most effective for the inhibition of influenza-A virus. Based on these results, further studies are warranted to evaluate the use of YQS extracts as a possible therapy for influenza.

並列關鍵字

fingerprint ； influenza-A virus ； Yinqiaosan