Experimental Study on Effect of Compounds in Inhibiting HCT-116 Human Colon Cancer Cells: The Preliminary Results

藥物對HCT-116人類大腸癌細胞株生長抑制的實驗：初步結果報告

張伸吉(Sheng-Chi Chang) ； 黃健財(Chien-Tsai Huang) ； 黃思銘(Ssu-Ming Huang)

《中華民國大腸直腸外科醫學會雜誌》 21卷3期 (2010/09) Pp. 121-129

https://doi.org/10.6312/SCRSTW.2010.21(3).09901

摘要

目的　分析數種藥物對於HCT-116人類大腸癌細胞株生長抑制的影響。
材料及方法　一些具有抗發炎或抗氧化或具有自由基清除作用之藥物被選用，包括厚朴酚(honokiol)，大黃素(emodin)，硫辛酸(lipoicacid)，黃連素(berberine)，皂甘(diosgenin)，白藜蘆醇(resveratrol)，呂宋揪莢粉素(rottlerin)，pinolo，薑黃素(curcumin)，退黑激素(melatonin)，以及丁酸鈉(sodiumbutyrate)。把HCT-116細胞培養在無血清環境之培養盤，分別加入上述藥物，分別以不同藥物濃度之條件下，於百分之五的二氧化碳濃度處理二十四或四十八小時。接著，再以3-[4,5,-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay分析HCT-116細胞生長受影響的情形。
結果　厚朴酚、大黃素、白藜蘆醇、呂宋揪莢粉素、薑黃素，這些藥物對於HCT-116細胞生長有明顯的抑制效果。經藥物處理四十八小時後，百分之五十的生長壓制劑量分別是厚朴酚爲18.5μM，大黃素爲17.3μM，白藜蘆醇爲25.3μM，呂宋揪莢粉素爲6.9μM，薑黃素爲22.3μM。
結論　厚朴酚、大黃素、白藜蘆醇、呂宋揪莢粉素、薑黃素，這些藥物對於HCT-116細胞生長有明顯的抑制效果。對於已篩選出的這些藥物，應當設計進一步的實驗探討牽涉在其中的機轉。

關鍵字

增生 ； 大腸癌 ； MTT分析

並列摘要

Objective. Several compounds were studied for their growth inhibitory effects on cultured HCT-116 human colon cancer cells.
Materials and Methods. Compounds with anti-inflammation, anti-oxidation, or free-radical scavenging ability were used, including honokiol, emodin, lipoic acid, berberine, diosgenin, resveratrol, rottlerin, pinolo, curcumin, melatonin, and sodium butyrate. Cultured cells were incubated in a serum-free medium with various concentrations of different compounds for 24 and 48 hours in a 5% CO2 incubator, after which the proliferation of HCT-116 cells was assessed by 3-[4,5,-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the significance of differences was analyzed by Student's t test.
Results. Honokiol, emodin, resveratrol, rottlerin, and curcumin used in this study were more effective than lipoic acid, berberine, diosgenin, pinolo, melatonin, and sodium butyrate. The 50% suppression doses after 48-hour exposure were 18.5 μM for honokiol, 17.3 μM for emodin, 25.3 μM for resveratrol, 6.9 μM for rottlerin, and 22.3 μM for curcumin respectively.
Conclusion. Further investigations should be conducted to elucidate the mechanisms modulating anti-tumor effects on HCT-116 cells for honokiol, emodin, resveratrol, rottlerin, and curcumin.

並列關鍵字

Proliferation ； Colon cancer ； MTT assay