植物提取物複方之面膜精華液其抗發炎之研究

Study on Anti-inflammatory Effect of Facial Mask Essence of Plant Extract Compound

林文宏(Lin, Wen-Hung)

《美容科技學刊》 18卷1期 (2021/06) Pp. 103-122

https://doi.org/10.30001/JIES.202106_18(1).006

摘要

本研究以植物提取物複方之面膜精華液，進行體外細胞抗發炎效果之測試，並對其安全性研究加以彙整。以植物提取物複方之面膜精華液， 利用巨噬細胞株細菌脂多醣（lipopolysaccharide, LPS）作用，分析三種發炎反應因子濃度，評估其抗發炎效果，包括一氧化氮（nitric oxide, NO）、介白素6（interleukin 6, IL-6）及腫瘤壞死因子-α（tumor necrosis factor-alpha, TNF-α），同時進行細胞毒性（microculture tetrazolium assay, MTT assay）分析。結果顯示，添加2.5 %植物提取物複方之面膜精華液，具顯著抗發炎反應，添加2.5%可抑制LPS誘發IL-6之生成量23.8%；對NO與TNF-α之生成則無顯著抑制作用。細胞毒性分析，在各受測濃度下，不具細胞毒性。綜上結果，植物提取物複方之面膜精華液，對於體外細胞層面之研究，呈現正效應。然，植物提取物複方之面膜精華液，整體之臨床治療效果，仍需更進一步研究。

關鍵字

植物提取物 ； 抗發炎 ； 細菌脂多醣

並列摘要

In this study the facial mask essence of plant extract compound was tested in vitro for cell anti-inflammatory effects, and compile its safety research. This study used this compound to analyze the concentration of three inflammatory response factors under the action of macrophage bacterial lipopolysaccharide (LPS) to evaluate its anti-inflammatory effect. This effect includes nitric oxide (NO), interleukin 6, IL-6, and tumor necrosis factor-alpha (TNF-α). Simultaneously, perform cytotoxicity (microculture tetrazolium assay, MTT assay) analysis. The results show that the addition of 2.5% plant extract compound mask essence can inhibit the production of IL-6 induced by LPS by 23.8%, and has a significant anti-inflammatory response; it has no significant inhibitory effect on the production of NO and TNF-α. In terms of cytotoxicity analysis, there is no cytotoxicity at all tested concentrations. In summary, the facial mask essence of plant extract compound shows a positive effect on in vitro cell-level. However, the overall clinical therapeutic effect of the facial mask essence of plant extract compound still needs further research.

並列關鍵字

plant extracts ； anti-inflammatory ； lipopolysaccharide ； LPS

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