- 期刊
The Effect of Auxin and Nitric Oxide on Root Hair Formation in Rice
生長素與一氧化氮對水稻根毛形成之影響
摘要
根毛具有吸收水分與養分及增加植株固著於土壤中的能力。因此，充分瞭解根毛發育之調控在農藝上有其重要性。本研究主要是探討生長素與一氧化氮對水稻根毛形成之影響。處理 sodium nitroprusside（SNP，一氧化氮釋放劑）與indole-3-butyric acid（IBA，一種植物體中存在的生長素）皆會誘導根毛的形成。一氧化氮清除劑2-（4-carboxy-2-phenyl）-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide（cPTIO）可抑制SNP 與IBA 所誘導的根毛形成。以螢光探針4-amino-5-methylamino-2, 7-difluorofluoresceindiacetate 來偵測內生一氧化氮量，SNP 與IBA 所誘導一氧化氮之螢光，可被cPTIO 抑制。我們的資料顯示IBA 所誘導之一氧化氮形成，主要是影響硝酸鹽還原酵素途徑所致。再者，SNP 與IBA 誘導一氧化氮形成的部位位於根毛的形成處。整體而言，我們的結果顯示生長素是經由一氧化氮來誘導水稻根毛之形成。
並列摘要
Root hair development performs the essential tasks of providing water, nutrients, and physical support to plants. Therefore, understanding the regulation of root hair development is of agronomic importance. In this study, we examined the effect of auxin and nitric oxide (NO) on root hair formation in rice. Treatment with sodium nitroprusside (SNP, a NO donor) or indole-3-butyric acid (IBA, a naturally occurring auxin) induced root hair formation and elongation. Root hair formation induced by SNP and IBA was reduced by the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline- 1-oxyl-3-oxide (cPTIO). Endogenous NO was detected by the specific fluorescence probe 4-amino-5-methylamino-2'7'Auxin, Nitric oxide, Rice, Root hairs- difluorofluorescein diacetate. Sodium nitroprusside and IBA were effective in inducing NO fluorescence, which was blocked by cPTIO. Results also showed that IBA-induced NO generation is primarily due to nitrate reductase (NR) in rice roots. Furthermore, NO generation caused by SNP or IBA was localized in root area corresponding to root hair emergence. Collectively, our data suggest that NO is responsible for auxin-induced root hair formation in rice.