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Identification of Rapd Primers Linked to Leaf Rust Resistant Gene Lr51 Introgressed from Triticum speltoides to Common Wheat (Triticum aestivum L.)

並列摘要


Leaf rust is the most important disease of wheat all over the world. Geneticists and breeder are now extracting new leaf rust resistant genes from wild relatives of wheat. An important leaf rust resistant gene Lr51 has been transferred from Triticum speltoides to common wheat. Present research was carried out to develop Randomly Amplified Polymorphic DNA (RAPD) based marker for the leaf rust resistant gene Lr51. Out of seven RAPD primers used, one RAPD prime viz; GLA-09 was found which could be used reliably to keep track of Lr51 in wheat background. This primer (GLA-09) amplified a polymorphic DNA fragment of approximately 1100 bp in the wheat lines having the leaf rust resistant gene Lr51.

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