- 期刊
- OpenAccess
Development of an ELISA Method Detecting Strobilurin Fungicide through the Immune Magnetic Beads Collecting Samples
並列摘要
Trifloxystrobin, one of the strobilurin fungicides, is active as inhibitor of low toxicity and easy degradation against a wide range of fungal plant pathogens. In the experiment, Hydroxy from magnetic microsphere and amino of anti-Trifloxystrobin antibody could condensate to form Immune Magnetic Beads (IMB), which could specially capture trifloxystrobin in the food matrix and rapidly separated it through the magnetic field. An ELISA method (IMB-ELISA) was developed to detect the captured fungicide through examining the best reactive concentration, sensitivity, specificity, matrix effect and recovery rate. The half maximal inhibitory concentration (IC50) value obtained by the IMB-ELISA method was 2.28μg/mL (R2=0.9837) against 12.57μg/mL of conventional ELISA method (R2=0.9747). The recovery rates were 90.12-103.65% and the Coefficients of Variation (CVs) were in the range of 4.5-11.2%. In three kind of citrus samples, there was only low cross-reactivity in IMB-ELISA method by detecting matrix effects. Based on above experiment, the IMB-ELISA method was an efficient and sensitive method for Trifloxystrobin detection.
延伸閱讀
- 顏枝梅、張碧秋、周薰修（1994）。Establishment of Standard Analytical Methods for Determination of Pesticide Residues in Crops-Tests of Phoxim。藥物食品檢驗局調查研究年報，()，177-180。https://doi.org/10.6946/ASRNL.199409.0177
- 蘇智勇（1991）。Field Trials of Granulosis Virus and Bacillus thuringiensis for Control of Plutella xylostella and Artogeia rapae。中華昆蟲，11(2)，x+174-178。https://doi.org/10.6660/TESFE.1991020
- 顏枝梅、張碧秋、周薰修（1994）。Establishment of Standard Analytical Methods for Determination of Pesticide Residues in Crops-Tests of Dicloran。藥物食品檢驗局調查研究年報，()，181-184。https://doi.org/10.6946/ASRNL.199409.0181
- 葉士財、謝慶昌（2015）。Study on the Fungicide Screening and Ultraviolet-C Irradiation Efforts for Inhibiting Neoscyralidium dimidiatum。臺中區農業改良場研究彙報，(127)，1-14。https://www.airitilibrary.com/Article/Detail?DocID=P20130930003-201506-201508180009-201508180009-1-14
- 顏枝梅、張碧秋、周薰修（1994）。Establishment of Standard Analytical Methods for Determination of Pesticide Residues in Crops-Tests of Phosphamidon。藥物食品檢驗局調查研究年報，()，173-176。https://doi.org/10.6946/ASRNL.199409.0173