An Epstein-Barr Virus Transformed Lymphoblastoid Cell Line Produces IgA Anti-HBsAg Human Antibody

一個EB病毒轉型之淋巴細胞瘤細胞株製造抗HBsAg之人類IgA抗體

曾哲明(Jer-Ming Tseng) ； 黃俊奇(Jung-Chi Hwang) ； 周千慧(Chin-Hui Chow)

《師大生物學報》 29卷2期 (1994/12) Pp. 107-111

https://doi.org/10.29812/BBNTNU.199412.0006

摘要

分離自抗HBsAg抗体陽性捐血者血液之單核細胞，在離体狀態下以HBsAg刺激後，再以Epstein-Barr病毒轉型為細胞株，穩定生長之後再選殖出具有製造抗HBsAg抗体之細胞株。其中有一子株〔DF4.22〕可在離体狀態下穩定分裂，並持續製造抗HBsAg之IgA抗体，以免疫熒光法檢試，發現DF4.22表面只有surface IgA，沒有surface IgG或IgM。此外， 91.3%細胞有MHC Class II, 30%有CD34，19.5%有CD21，顯示此淋巴細胞瘤細胞株維持在pre-B細胞狀態，且CD21〔EB病毒受体〕之表現被抑低了。

關鍵字

淋巴瘤細胞株 ； 抗HBsAg抗體

並列摘要

Peripheral blood mononuclear cell from anti-HBsAg antibody seropositive donors were in vitro challenged with HBsAg, transformed with the Epstein-Barr virus and cloned for production of anti-HBsAg antibody. One of the subclones, DF4.22, showed stable growth, constantly produced IgA anti-HBsAg antibody and expressed surface IgA but did not express IgG or IgM. The DF4.22 cells were MHC Class 11+ (91.3%), CD34+ (30%) and CD21+ (19.5%), suggesting that the lympho-blastoid cells are in pre-B cell stage and the CD21 (Epstein-Barr Virus receptor) on DF4.22 was down-regulated.

並列關鍵字

Lymphoblastoid cells ； Anti-HBsAg