逆相層析多柱系統選擇性規律研究

本文以烷基苯、氯化苯、硝基取代芳香化合物、多環芳烴四類化合物分別在C_(18)、C_8、苯基三種商用管柱上作層析分離，綜合各管柱最佳分離選擇性互補情況，研究逆相層析多柱系統選擇性之規律，得出：對於非極性或弱極性的同類難分離檢品對，應選擇固定相極化率盡可能大的管柱。對於強極性難分離檢品對，可藉由增加或降低固定相極化率而選擇另一管柱，以達到難分離檢品對的分離。對於像多環芳煙這樣較為複雜的某一管柱上的難分離檢品對，可選擇不同極化率、鍵合量或其他性質不同的另一管柱作為第二柱。對於不同類檢品間，結構上存在一定差別而在一管柱上難分離的檢品對，可選取另一作用力有差別的管柱作為第二柱。對於改變固定相極化率後，仍不能解決其分離問題的難分離磁品對，應選擇有特殊相互作用的另一管柱以形成互補分離。

關鍵字

逆相層析；多柱系統；選擇性

並列摘要

This research is the study of the chromatographic separation of four kinds of compounds (alkylbenzenes , chlorobenzenes, nitobenzenes and PAHs) on three commercial columns (C_(18), C_8 and PHE), and the regularity in the reverse phase condition. This condition is studied by using the best, optimized and mutual characteristics of selectivity of each column. Results show: for non-polar or weak polar compounds of similar types which are hard to separate one should choose stationary phases of great polarity which are as large as possible. The strong polar compounds of similar types may either increase or decrease the polarity of the stationary phase when choosing another column to obtain better select ivity. The PAHs which are difficult to separate on the first column, can also be applied to another column with characteristics of different polarity, bonding numbers, or other properties different from the first column . As for structurally different samples and poorly separated on the first column, the column with the predicted different interactions with compounds, might be a candidate for the second column. In the case of choosing different polarity in the second column, as compared to the first column, but does not obtain sati sfactory separation, special mutual interaction should be considered.

並列關鍵字

Reverse Phase Chromatography ； Multi-Column System ； Selectivity

國際替代計量

逆相層析多柱系統選擇性規律研究

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