六種花來源純露之組成分析及清除DPPH自由基能力

Composition analysis and DPPH radical scavenging activity of six hydrosols obtained from flowers

許儷齡(Li-ling Syu) ； 徐照程(Jaw-Cherng Hsu)

《弘光學報》 71期 (2013/09) Pp. 40-54

https://doi.org/10.6615/HAR.201309.71.04

摘要

本研究以氣相層析質譜儀(Gas Chromatography-Mass Spectrophotometer，GC-MS)分析野薑花、德國洋甘菊、台灣茉莉花、玉蘭花、玫瑰、柚子花等六種花來源純露(Hydrosol)的組成，並探討其清除DPPH自由基之抗氧化能力與紫外線吸收能力。純露以分流比1：100的方式直接注入GC-MS，配合初溫70℃，每分鐘5℃升溫速率至150℃，停留5分鐘，再以每分鐘5℃加溫至250℃的溫度梯度沖提，可在45分鐘內有效分析純露，並藉由比對質譜資料庫與文獻記錄，鑑定出其中主要成份。分析結果六種純露中以醇類與氧化物的含量最多，如玫瑰醇露組成中醇類的含量高達95.09%，野薑花醇露組成中的氧化物含量則有66.64%。DPPH自由基清除能力的實驗結果顯示較佳者為100%野薑花純露與100%玫瑰純露，清除DPPH自由基之能力分別為73.95%、68.8 4%。與其化學組成比對，野薑花純露含有66.64%的桉油醇(Cineole)與1.34%的異丁香酚(Isoeugenol)為其抗氧化成分；而玫瑰純露含有1.09%丁香酚(Eugenol)，具有抗氧化抗自由基等功效。此外，將純露於定量濃度下使用紫外光-可見光分光光譜儀評估純露的紫外光吸收能力。實驗結果發現，德國洋甘菊與柚子花純露在290～400nm處有較佳的吸收值，預期未來添加在化妝品中能達到輔助防曬的效果。

關鍵字

純露(Hydrosol) ； 氣相層析質譜儀(GC-MS) ； DPPH ； 抗氧化(antioxidant)

並列摘要

In this study, the hydrosol of White ginger lily (Hedychium coronarium ), Chamomile (Matricaria chamomilla ), Jasmine (Jasminum officinale ), White Champaca (Magnolia × alba ), Damask rose (Rosa × damascena ) and Pomelo (Citrus maxima ) have been analyzed by GC-MS and tested for their antioxidant and UV protective effectiveness. Compounds were identified by comparing the retention times and retention indices of the chromatographic peaks and by mass spectral database search followed by matching of MS data. The major class of compounds found in six kind of hydrosol is alcohol and oxide, Damask rose hydrosol contains 95.09% alcohol, White ginger lily hydrosol contains 66.64% oxide. White ginger lily and Damask rose hydrosol shows the highest DPPH radical scavenging ability, White ginger lily hydrosol contains 66.64% Cineole as the potent scavenging compound. The UVB and UVA absorbing properties of Chamomile and Pomelo show potential as SPF booster in sunscreens formulation.

並列關鍵字

Hydrosol ； GC-MS ； Antioxidant Activity ； Flower

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