Detam 1 black soybean against cisplatin-induced acute ren failure on rat model via antioxidant, antiinflammatory and antiapoptosis potential

Wahyu Widowati ； Sijani Prahastuti ； Meilinah Hidayat ； Stella Tinia Hasianna ； Roro Wahyudianingsih ； The Fransiska Eltania ； Alya Mardhotillah Azizah ； Jenifer Kiem Aviani ； Mawar Subangkit ； Rr Anisa Siwianti Handayani ； Hanna Sari Widya Kusuma

《Journal of Traditional and Complementary Medicine》 12卷4期 (2022/07) Pp. 426-435

https://doi.org/10.1016/j.jtcme.2022.01.004

摘要

Background and aim: Cis-Diamminedichloroplatinum (II) (Cisplatin) is one of the most synthetic anticancer drug but have several adverse effects and one of them is acute ren failure. Cisplatin can induce nephrotoxicity occur via the toxic generation of reactive oxygen species (ROS). Black soybean (Glycine max L. Merr.) has been reported contain high levels of phenolics and anthocyanins that has antioxidant activity. This study aims to determine the effect of ethanol extract of black soybean (EEBS) against cisplatin-induced nephrotoxicity in rats. Experimental procedure: Cisplatin-induced nephrotoxicity rats treated with EEBS and the blood samples taken on days 0, 9, and 18. The effects of EEBS was evaluated by determining Interferon-γ (IFN-γ), Caspase-3 (Casp-3), and Interleukin-1β (IL-1β) expression using immunohistochemistry (IHC), blood urea nitrogen (BUN), Uric Acid (UA) content and catalase (CAT) content in the blood plasma with colorimetric assay kit. Results and conclusion: Based on the results, EEBS treatment had successfully reduced pro-inflammatory cytokines IL-1βand IFN-γ, and improved physiological condition by lowering BUN and UA content while increasing CAT activity. No significant effect was found in Casp-3 expression. EEBS has potential to improve acute renal failure condition through inflammatory suppression and renal function improvement.

關鍵字

Blood urea nitrogen ； Catalase ； Fibronectin ； Glycine max L. Merr. ； Nephrotoxicity

參考文獻

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