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使用PLGA骨板固定大鼠實驗性股骨骨折之研究

Fixation Effects of PLGA Plate in Experimental Femora Bone Fracture of Rats

摘要


本研究的目的在於利用PLGA(Poly Lactic-co-Glycolic Acid)製成的骨板,將其應用於固定實驗性老鼠股骨骨折,並將之與鈦合金骨板之固定效果作一比較。本實驗以20隻10週大,體重約500克重的S/D Rat,隨機分成實驗組及對照組(每組各10隻),於全身麻醉和無菌狀態下,以手術方法曝露出股骨,並以高速手機配合直徑0.1公分直型鑽針,由股骨外側鋸開至內側,造成不完全骨折(內側之皮質骨不鋸斷)。而實驗組股骨之骨折以PLGA骨板固定,對照組則以鈦合金骨板固定。於術後20天及40天將老鼠犧牲,並取出股骨,脫鈣後,以臘包埋,以5μm厚度切片後,採H&E染色並進行組織形態之觀察。結果發現以鈦合金骨板固定的老鼠股骨傷口癒合良好,而以PLGA骨板固定的股骨則易導致感染,且於皮下產生大量的膿塊。以鈦合金骨板固定的老鼠股骨皆沒有骨折現象,而以PLGA骨板固定的股骨香生變形且趨於骨折。於組織切片觀察下,鈦合金骨釘被大量成熟骨包圍,且極為緻密,而PLGA骨釘周圍則產生大量的纖維組織,且於纖維組織外可發現大量軟骨生成。以鈦合金骨板固定的股骨切口癒合良好,以PLGA骨板固定的股骨切口處則生成大量纖維組織。顯示Lactosorb PLGA骨板系統與鈦合金骨板系統相比較,PLGA骨板系統並不適用於固定老鼠股骨實驗性骨折。

並列摘要


The aim of the study is to use the PLGA plates to fix the experimental femur fracture of rats, and compared that with the titanium plates. At the beginning, twenty 10 weeks old, with mean body weight of 500 gm S/D rats were randomly divided into two groups, one experiment and the other control groups. Under general anesthesia, after antiseptic, the femur was exposed, and the femur was cut from the outer cortical bone till the inner cortical bone (with the inner cortical bone intact) using dental high speed with fissure bur (diameter 0.1 cm). PLGA plates were used to fix the fractured femur in experiment group while titanium plates were used for the control group. 20 and 40 days after the surgery, the femur was removed, embedded with paraffin wax after demineralized. The specimens was cut with 5μm thickness and undergoing histological observation after H & E staining. The results showed that the femur fixed with titanium plate healed very well. The femur fixed with PLGA plates tends to get infection and there was a lot of pus formation around the wound. Under histological observation, there was a lot of fibrotic tissue around the screws of PLGA and a lot of cartilages formation next to the defect of the femur. On the other hand, there was dense bone formation around the screws of titanium. There was no fracture of the femur with titanium fixation while the femur fixed with PLGA plates tends to get fracture. The study shows that the Lactosorb PLGA plates are not suitable for fixation over the experimental femoral fracture of the rats.

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