- 期刊
微生物製劑蘇力菌以色列變種對鵪鶉口服之安全性
Safety Evaluation of Bacillus Thuringiensis Var. Israelensis for Japanese Quail by Oral Administration
摘要
以純化的微生物製劑蘇力菌以色列變種（Bacillus thuringiensis var. israelensis）對本土性的16日齡鵪鶉（Japanese quail; Coturnix coturnix）進行口服安全試驗評估，以暸解其對鳥類的毒性、感染性及在體內分佈、停留之情形。檢查內容包括糞材及組織臟器之回收培養、每週飼料消耗量、鵪鶉的體重變化、血液、血清生化及病理檢查等。蘇力菌孢子懸浮液連續以胃管方式投於5天後，於第35天解剖。結果發現蘇力菌孢子大部份由消化道途徑排出，投於後之前5天為清除高峰期，其後漸減，唯可持續越35天。臟器塗抹培養結果未發現孢子的分佈。飼料消耗量、動物的體重、血液學；ALT（GPT）、AST（GOT）、BUN、glucose及total protein等血清生化檢驗及病理病變，試驗組與對照組無明顯差異。此結果顯示國內製造之蘇力菌以色列變種對鳥類無明顯的毒性、感染性及致病性，本試驗結果為提供微生物製劑對鳥類安全評估重要模式。
並列摘要
In the present study, avian safety evaluations were performed, wherein preparation of Bacillus thruingienisis var. israelensis (Bti) was followed by the oral administration of the same to Japanese quail (Coturnix coturnix). The toxicity, infectivity and pathogenicity of B. thuringiensis towards Japanese quail were evaluated. Quails were first orally inoculated with suspension spores of Bti for a period of five days. Then, the bacteria spore culture obtained from faces and organ tissues at various time intervals were identified. In quails the main way of Bti clearance following the oral intake is through the digestive system. The spores of Bti in feces were mostly detected within 5 days, which further decreased in 9 days, whereas in quail feces, they lasted a period of for over 35 days after administration of the dose. Bti spore culture, however could not be detected in the organ tissues and blood. The food consumption and changes in body weight of Japanese quail in the treated group showed no significant difference as compared to the control group. Furthermore, the hematology, biochemistry and gross findings in treated groups also exhibited no significant changes as compared to control groups. Neither any clinical signs nor a significant histopathological change was found during the test. The present results confirmed the safety of Bacillus thuringiensis var. israelensis in quails.