- 期刊
Humoral Immune Responses in Mice Infected with Gamma-Irradiated Third-Stage Larvae of Angiostrongylus Cantonensis
經伽傌線照射的廣東住血線蟲第三期幼蟲感染小鼠後誘發之抗體免疫反應
摘要
為瞭解C57BL6（B6）品系小鼠感染對經伽傌射線照射的廣東住血線蟲第三期幼蟲後之免疫反應，以經伽傌射線及未照射的第三期幼蟲感染後，連續觀察十週，就兩者與宿主免疫反應關係，作一探討。感染兩者後小鼠的血清特異性抗體，從感染後第一週逐漸升高，於第三週達到高峰，以後則呈逐週下降的現象；利用血清抗體認知抗原成分的測定中，經伽傌射線照射的第三期幼蟲與未經伽傌射線照線的第三期幼蟲感染的B6小鼠，二者幾乎無差異變化，在感染後早期的小鼠血清均能認知第五期幼蟲蟲體抗原分子量在47，110及204KD的部分；在抗體依賴慌嶠血球對廣住血線蟲幼蟲的吸附效應中，二者的差異亦不大，以感染後第三週抗體的作用最明顯，其吸附效應最高；在T細胞依賴性抗體對綿羊紅血球的成斑細胞實驗中，二者在感染早期，其成斑細胞的形成均受到抑制。
關鍵字
無資料並列摘要
Humoral immune responses in C57BL/6 strain mice ifected with 1.0 kGy gamma-irradiated third-stage larvae (L3) and with non-gamma-irradiated L3 of Angiostrongylųs cantonensis were assessed in this study. The young-adult worm (L5) antigen-specific antibodies in sera of both groups of mice elevated gradually and reached a peak in the third week after infection. The IgM antibodies of mice 1-week after infection with gamma-irradiated L3 and with non-gamma-irradiated L3 recognized several L5 antigens of the same molecular weight. However, only partially identical profiles were observed in the reaction of L5 antigens with IgM and IgG antibodies from mice 3 weeks after infection with gamma-irradiated L3 and with non-gamma-irradiated L3 respectively by western blot analysis. With regard to antibody-dependent cell-mediated cytotoxicity, antibodies from 3-week infected mice significantly increased the adherence of mouse eosinophils and neutrophils to L3 of A. cantonensis. The immunoresponses of splenic cells from mice in the third week of infection to sheep red blood cells were suppressed because the number of plaque forming cells obviously decreased more than the uninfected control.