- 期刊
- OpenAccess
Biomedical Availability of β-Tricalcium Diphosphate Converted from Oyster Shells
摘要
The oyster shells were converted into a biomedical-related compound, β-tricalcium phosphate (β- Ca_3(PO_4)_2, β-TCP), using wet chemical precipitation. The crystal phase and chemical structure of β-TCP analogue were analyzed by X-ray diffractometry (XRD) and Fourier transform infrared spectrometry (FTIR), respectively. In XRD pattern, there have the same four significant peaks between the diffraction peaks of 25 - 35° in the β-TCP analogue converted from oyster shell as those of commercial product. In FTIR pattern, the absorption peaks of β-TCP analogue converted from oyster shell are the same as those of the commercial product, exhibiting peaks of 1025 cm^(-1) and 601 cm^(-1) for the asymmetric stretching vibration peak of PO_4^(3-) and the bending vibration peak, respectively. Furthermore, the biomedical activity of β-TCP analogue was determined using osteoblasts (MG-63 cells) in vitro cell culture of osteoblasts (MG-63 cells). The cell viability was better and equal to that of commercial product. The alkaline phosphatase analysis showed the cell differentiation was significant effective and equal to that of commercial products. Alizarin red staining assay showed that the cell mineralization was significantly higher and equal to that of commercial product. Therefore, the availability of β-TCP analogue produced from oyster shells is good as commercial product.